Keep Current with the Latest in Cell Biology Research

Identifying SARS-CoV-2 Variants Using Single-Molecule Conductance Measurements

Researchers demonstrated that RNA-based, single-molecule conductance experiments could be used to identify specific variants of SARS-CoV-2. [ACS Sensors] Abstract

Human iPSC and CRISPR Targeted Gene Knock-In Strategy for Studying the Somatic TIE2L914F Mutation in Endothelial Cells

[Angiogenesis] To study the effects of targeted mutations on an endogenous level, scientists generated and characterized an iPSC derived model for venous malformations (VMs). CRISPR-Cas9 technology was used to generate a novel human iPSC line with an amino acid substitution L914F in the TIE2 receptor, known to cause VMs.

Superior Induced Pluripotent Stem Cell Generation through Phactr3-Driven Mechanomodulation of Both Early and Late Phases of Cell Reprogramming

[Biomaterials Research] Researchers found that a medium stiffness gel increased the overall number of reprogrammed cells by up to 10-fold, accelerated reprogramming kinetics and produced iPSCs having more naïve characteristics and lower remnant transgene expression, compared to the gold standard tissue culture polystyrene practice.

Ultrasound-Triggered Three Dimensional Hyaluronic Acid Hydrogel Promotes In Vitro and In Vivo Reprogramming Into Induced Pluripotent Stem Cells

[Bioactive Materials] Scientists presented a new reprogramming strategy using low-intensity ultrasound to improve cellular reprogramming of iPSCs in vitro and in vivo.

Generation of Induced Pluripotent Stem Cell Lines From Patients With LQT1 Caused by Heterozygous Mutations in the KCNQ1 Gene

[Stem Cell Research] Investigators generated two LQT1 iPSC lines with single nucleotide nonsense mutations, c.1031 C > T and c.1121 T > A in KCNQ1. Both lines exhibited typical iPSC morphology, expressed high levels of pluripotent markers, maintained normal karyotype, and possessed the capability to differentiate into three germ layers.

Generation of Induced Pluripotent Stem Cells From an Individual with Early Onset and Severe Hypertrophic Cardiomyopathy Linked to MYBPC3: c.772G > a Mutation

[Human Cell] Researchers generated two iPSC lines from peripheral blood mononuclear cells of a female with early onset and severe hypertrophic cardiomyopathy linked to the MYBPC3: c.772G > A variant.

Establishing a Human-Induced Pluripotent Stem Cell Line SMUSHi005-A From a Patient with Hypophosphatemic Vitamin D-Resistant Rickets Carrying the PHEX c.1586–1586+1 delAG Mutation

[Stem Cell Research] The SMUSHi005-A hiPSC line was successfully established from the peripheral blood mononuclear cells of a female patient carrying the PHEX mutation with c.1586–1586+1 delAG. The iPSC line has been confirmed to have a normal karyotype.

Epigenetic Inheritance of Acquired Traits via Stem Cells Dedifferentiation/Differentiation or Transdifferentiation Cycles

[Cells & Development] The authors hypothesize existence of pluripotent adult stem cells that can serve as collectors and carriers of new epigenetic traits by entering different developmentally active organ/tissue compartments through blood circulation and acquiring new epigenetic marks though cycles of differentiation/dedifferentiation or transdifferentiation.

Parkinson’s Patient Moves Freely Again after World-First Implant of Lab-Grown Cells Into His Brain

[STEM-PD] Researchers at Lund University have successfully implanted 7 million lab-grown brain cells into a patient to treat Parkinson's disease. Swedish resident Thomas Matsson was the first in the world to test the method about a year ago

Characterization of Novel Influenza-Derived HLA-B*18:01-Restricted Epitopes

[Clinical & Translational Immunology] Scientists assessed the immunogenicity of six recently published novel influenza-derived peptides identified by mass-spectrometry and predicted to bind to the prevalent HLA-B*18:01 molecule.

The EBI2 Receptor Is Coexpressed with CCR5 in CD4+ T Cells and Boosts HIV-1 R5 Replication

[AIDS] Investigators looked for G protein-coupled receptors (GPCRs) able to modify HIV-1 infection. They identified GPCRs expressed in primary CD4+CCR5+ T cells by multi-RT-qPCR.

STAT1 Regulates Neutrophil Gelatinase B-Associated Lipocalin Induction in Influenza-Induced Myocarditis

[Scientific Reports] Scientists investigated the role of anti-viral STAT1 signaling in influenza-induced myocarditis. Wild-type mice (C57BL/6) were infected with either influenza A/PR/8/34 or control, and cellular response and gene expression analysis from the heart samples were assessed 7 days later.

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