Keep Current with the Latest in Cell Biology Research

DNMT3B Supports Meso-Endoderm Differentiation from Mouse Embryonic Stem Cells

[Nature Communications] Using in vitro stem cell differentiation and loss of function experiments combined with high-throughput genome-wide bisulfite-, bulk-, and single cell RNA-sequencing, researchers dissected the specific role of DNMT3B in cell fate.

Epigenetic and Transcriptional Regulations Prime Cell Fate before Division during Human Pluripotent Stem Cell Differentiation

[Nature Communications] Using the FUCCI reporter, scientists developed a cell-cycle synchronized hPSC differentiation system for uncovering epigenome and transcriptome dynamics during the first two divisions leading to definitive endoderm.

Lnc956-TRIM28-HSP90B1 Complex on Replication Forks Promotes CMG Helicase Retention to Ensure Stem Cell Genomic Stability and Embryogenesis

[Science Advances] Investigators identified an uncharacterized long noncoding RNA NONMMUT028956 (Lnc956) predominantly expressed in mESCs. Lnc956 was accumulated on replication forks to prevent fork collapse and preserve genomic stability and was essential for mouse embryogenesis.

RNA Exosome Ribonuclease DIS3 Degrades Pou6f1 to Promote Mouse Pre-implantation Cell Differentiation

[Cell Reports] To overcome the paucity of embryos, the authors derived homozygous Dis3 null mESCs to identify additional gene targets of POU6F1.

Efficient Generation of Marmoset Primordial Germ Cell-Like Cells Using Induced Pluripotent Stem Cells

[Elife] Researchers established robust methods for iPSC culture in the common marmoset, allowing stable propagation in an undifferentiated state.

NANOGP1, a Tandem Duplicate of NANOG, Exhibits Partial Functional Conservation in Human Naïve Pluripotent Stem Cells

[Development] To study the contribution of duplicated genes to human early development, investigators examined the evolution and function of NANOGP1, a tandem duplicate of the transcription factor NANOG.

FAM122A Is Required for Mesendodermal and Cardiac Differentiation of Embryonic Stem Cells

[Stem Cells] Scientists observed that Fam122a deficiency impaired mesendodermal specification and cardiac differentiation from mESCs but showed no influence on pluripotent identity.

Expression of Chrna9 Is Regulated by Tbx3 in Undifferentiated Pluripotent Stem Cells

[Scientific Reports] The authors comprehensively investigated the gene expression of α subunit of nicotinic acetylcholine receptors (Chrna) during differentiation and induction of PSCs. mESCs expressed multiple Chrna genes in undifferentiated status.

Nanosheet Coating Improves Stability of Human Pluripotent Stem Cell Culture on Glass Substrates

[Biochemical and Biophysical Research Communications] Investigators reported that coating the glass substrate using nanosheet composed of hydrophobic polystyrene, with Matrigel, significantly improved the viability of hPSCs.

Characterization of Human Induced Pluripotent Stems Cells: Current Approaches, Challenges, and Future Solutions

[Biotechnology Reports] Scientists review some of the key analytical methods used as part of identity, potency, or safety in process or final product release testing and highlighted the challenges and potential solutions for consideration in the chemistry, manufacturing, and controls strategy for iPSC-based therapies.

Two KI Researchers Awarded ERC Consolidator Grants

[Karolinska Institute] Two Karolinska Institute researchers – Simon Elsässer and Magda Bienko – had been awarded 2022 European Research Council (ERC) Consolidator Grants that will support two ambitious basic research projects on understanding of the complex nature of cells.

Low-Dose Intravenous and Subcutaneous CIS43LS Monoclonal Antibody for Protection against Malaria (VRC 612 Part C): A Phase I, Adaptive Trial

[Lancet Infectious Diseases] Adults aged 18–50 years with no previous malaria vaccinations or infections received the monoclonal antibody CIS43LS in a single, open-label dose of 1 mg/kg, 5 mg/kg, or 10 mg/kg intravenously, or 5 mg/kg or 10 mg/kg subcutaneously.

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