lbeveridge

[Nature Microbiology] Scientists investigated P. aeruginosa growth and antibiotic tolerance in tissue-engineered airways by transposon insertion sequencing. Metabolic modelling based on Tn-seq data revealed the nutritional requirements for P. aeruginosa growth, highlighting reliance on glucose and lactate and varying requirements for amino acid biosynthesis.

[PharmaPhorum] MSD and Moderna have kicked off another Phase III trial of their personalised cancer vaccine V940 used alongside MSD's PD-1 inhibitor Keytruda, this time focusing on patients with earlier-stage NSCLC.

[Scientific Reports] Stable, small size cationized extracellular vesicles were loaded with plasmid DNA, or mRNA, or siRNA. Nucleic acid loaded extracellular vesicles were efficiently taken up by target cells as demonstrated by confocal microscopy and delivered their cargo to the nuclei in triple negative breast cancer cells and macrophages

[Cell Reports Medicine] Scientists reported a patient with isolated idiopathic LEMS treated with autologous anti-CD19-CAR-T cells. In this patient, CAR-T infusion led to expansion of predominantly CD4+ CAR-T cells with a terminally differentiated effector memory cells re-expressing CD45RA-like phenotype indicating cytotoxic capabilities and subsequent B cell depletion.

[Nature] Investigators analyzed hematopoietic reconstitution in 53 patients treated with lentiviral-HSC-gene therapy for diverse conditions such as metachromatic leukodystrophy2,3, Wiskott-Aldrich syndrome4,5, and β-thalassemia6 over a follow-up period of up to eight years, using vector integration sites as markers of clonal identity.

[Science Advances] The authors investigated the effects of matrix-bound nanovesicles (MBVs) on RANKL-induced osteoclast formation in vitro and particulate-induced osteolysis in vivo. Results showed that MBV attenuated osteoclast differentiation and activity by suppressing the NF-κB signaling pathway and downstream NFATc1, DC-STAMP, c-Src, and cathepsin K expression.