Sarcomeric Tropomyosin Expression during Human iPSC Differentiation into Cardiomyocytes

During the differentiation time course, researchers harvested cells on days 0, 5, 10, 15, and 20 to analyze for various sarcomeric tropomyosin (TPM) transcripts by qRT-PCR and for sarcomeric TPM proteins using two-dimensional Western blot with sarcomeric TPM-specific CH1 monoclonal antibody followed by mass spectra analyses.