Inflammation-Driven Deaminase Deregulation Fuels Human Pre-Leukemia Stem Cell Evolution

Comparative whole-genome and whole-transcriptome sequencing analyses of FACS-purified pre-leukemia stem cells from myeloproliferative neoplasm patients reveal APOBEC3C upregulation, an increased C-to-T mutational burden, and hematopoietic stem and progenitor cell proliferation during progression, which can be recapitulated by lentiviral APOBEC3C overexpression.
[Cell Reports]
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Tumor Cell Network Integration in Glioma Represents a Stemness Feature

Glioblastoma cells that are part of the tumor microtube-connected tumor network showed activated neurodevelopmental and glioma progression gene expression pathways. Importantly, many of them revealed profiles indicative of increased cellular stemness, including high expression of nestin.
[Neuro-Oncology]
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Organoid Sample Preparation and Extraction for LC-MS Peptidomics

Scientists describe the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry.
[STAR Protocols]
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Lithocholic Bile Acid Induces Apoptosis in Human Nephroblastoma Cells: A Non-Selective Treatment Option

Scientists evaluated the effects of Lithocholic bile acid (LCA) on nephroblastoma. To test the effects of LCA, nephroblastoma cell line WT CLS1 was used.
[Scientific Reports]
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Autofluorescence-Based Sorting Removes Senescent Cells from Mesenchymal Stromal Cell Cultures

Investigators tested the suitability of MSC-sorting based on their FACS autofluorescence profile, for a rapid and non-invasive method of senescent cell elimination.
[Scientific Reports]
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Reshaping the Immune Microenvironment by Oncolytic Herpes Simplex Virus in Murine Pancreatic Ductal Adenocarcinoma

Researchers established an immunocompetent syngeneic pancreatic ductal adenocarcinoma model, and investigated the effect of oncolytic herpes simplex virus-1 on the composition of tumor microenvironment immune cells.
[Molecular Therapy]
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Metabolic Programming of Distinct Cancer Stem Cells Promotes Metastasis of Pancreatic Ductal Adenocarcinoma

Scientists investigated the role of distinct subtypes of pancreatic cancer stem cells and their metabolomic signatures in organ-specific metastatic colonization.
[Oncogene]
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TGFBI Modulates Tumor Hypoxia and Promotes Breast Cancer Metastasis

Researchers crossed the MMTV‐PyMT model of mammary gland tumorigenesis with a TgfbiΔ/Δ mouse, and studied the cancer stem cell (CSC) content of the tumors. They performed RNAseq on WT and KO tumours, and analyzed the tumor vasculature and the immune compartment by IHC and FACS.
[Molecular Oncology]
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Aggressive PDACs Show Hypomethylation of Repetitive Elements and the Execution of an Intrinsic IFN Program Linked to a Ductal Cell-of-Origin

Researchers FACS-purified epithelial cells from human pancreatic ductal adenocarcinoma and normal pancreas and derived their genome-wide transcriptome and DNA methylome landscapes.
[Cancer Discovery]
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Isolation and Enrichment of Melanocytes from Human Corneal Limbus Using CD117 (C-Kit) as Selection Marker

Investigators report on a facile protocol for the efficient isolation and enrichment of pure populations of human limbal melanocytes by fluorescence-activated cell sorting using antibodies raised against the cell surface marker CD117.
[Scientific Reports]
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Establishment and Validation of an In Vitro Co-Culture Model for Oral Cell Lines Using Human PBMC-Derived Osteoclasts, Osteoblasts, Fibroblasts and Keratinocytes

Researchers investigated the viability and proliferation of osteoblasts, fibroblasts and oral keratinocytes under stratified medium modification and assessed the differentiation of osteoclasts in each co-culture.
[Scientific Reports]
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