Loss of PTEN in fallopian tube epithelium led to the enrichment of CSC markers such as LGR5, WNT4, ALDH1, CD44.
[Cell Death & Disease]
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Russo, A., Colina, J. A., Moy, J., Baligod, S., Czarnecki, A. A., Varughese, P., Lantvit, D. D., Dean, M. J., & Burdette, J. E. (2021). Silencing PTEN in the fallopian tube promotes enrichment of cancer stem cell-like function through loss of PAX2. Cell Death & Disease, 12(4), 1–14. https://doi.org/10.1038/s41419-021-03663-2 Cite
Elevation of Cdc42 activity in young animals by genetic means causes premature ISC aging, whereas pharmacological suppression of elevated Cdc42 activity restores organoid formation potential in vitro.
Colonoid and enteroid gene expression signatures were more similar to those found for the tissues of their origin than to each other.
[American Journal of Physiology-Cell Physiology]
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The authors studied the impact of chemotherapy on Lgr5-expressing and MSI1-expressing cell populations and explored the effect of increased MSI1 levels in response to treatment.
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Frau, C., Jamard, C., Delpouve, G., Guardia, G. D. A., Machon, C., Pilati, C., Neve, C. L., Laurent-Puig, P., Guitton, J., Galante, P. A. F., Penalva, L. O., Freund, J.-N., Fouchardière, C. de la, & Plateroti, M. (2021). Deciphering the role of intestinal crypt cell populations in resistance to chemotherapy. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-20-2450 Cite
Investigators proposed that geminin overexpression in normal mammary epithelial cells promoted the generation of TNBC metastatic precursors that home specifically to lungs by upregulating LGR5 expression and promoting stemness, intravasation, and extravasation in these precursors.
[Cancer Gene Therapy]
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Scientists showed that Twist1 had a direct role in maintaining the balance between proliferation and differentiation of keratinocytes and keratinocyte stem/progenitor populations.
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To determine the physiological role of MAP3K2-regulated intestinal stromal cells (MRISCs), scientists co-cultured MRISCs with colonic organoids and found that organoid growth was greater than when co-cultured with MRISC-depleted stromal cells
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Wu, N., Sun, H., Zhao, X., Zhang, Y., Tan, J., Qi, Y., Wang, Q., Ng, M., Liu, Z., He, L., Niu, X., Chen, L., Liu, Z., Li, H.-B., Zeng, Y. A., Roulis, M., Liu, D., Cheng, J., Zhou, B., … Su, B. (2021). MAP3K2-regulated intestinal stromal cells define a distinct stem cell niche. Nature, 1–5. https://doi.org/10.1038/s41586-021-03283-y Cite
The authors summarize currently available adult cell‐derived hepatobiliary organoid models and their applications.
The authors found that IL-17RB expression was significantly upregulated in spheroid cells and Lgr5-positive cells from the same tumor tissues of patients with gastric cancer, which was closely correlated with the degree of cancer cell differentiation.
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Bie, Q., Song, H., Chen, X., Yang, X., Shi, S., Zhang, L., Zhao, R., Wei, L., Zhang, B., Xiong, H., & Zhang, B. (2021). IL-17B/IL-17RB signaling cascade contributes to self-renewal and tumorigenesis of cancer stem cells by regulating Beclin-1 ubiquitination. Oncogene, 1–17. https://doi.org/10.1038/s41388-021-01699-4 Cite
Using a Lgr5-2A-DTR model, which ablates Lgr5+ cells with near-complete efficiency and retains endogenous levels of Lgr5 expression, researchers showed that persistent depletion of Lgr5+ intestinal stem cells in fact compromised small intestinal epithelial integrity and reduced epithelial turnover in vivo.
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Scientists found that recombinant EREG, BTC, and NRG1 but not Lgr5 ligand R-Spondin promoted growth and proliferation of Apc double mutant colonic organoids.
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Westendorp, F., Karpus, O. N., Koelink, P. J., Vermeulen, J. L. M., Meisner, S., Koster, J., Büller, N. V. J. A., Wildenberg, M. E., Muncan, V., & van den Brink, G. R. (2021). Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation. Oncogene, 1–16. https://doi.org/10.1038/s41388-020-01633-0 Cite