CREPT Is Required for Murine Stem Cell Maintenance during Intestinal Regeneration

Scientists report that CREPT, a recently identified tumor-promoting protein, was required for the maintenance of murine intestinal stem cells. CREPT was preferably expressed in the crypts but not in the villi.
[Nature Communications]
Yang, L., Yang, H., Chu, Y., Song, Y., Ding, L., Zhu, B., Zhai, W., Wang, X., Kuang, Y., Ren, F., Jia, B., Wu, W., Ye, X., Wang, Y., & Chang, Z. (2021). CREPT is required for murine stem cell maintenance during intestinal regeneration. Nature Communications, 12(1), 270. https://doi.org/10.1038/s41467-020-20636-9 Cite
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Establishment of Intestinal Organoid Cultures Modeling Injury-Associated Epithelial Regeneration

Researchers established a novel intestinal organoid culture system composed of 8 components, mainly including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury.
[Cell Research]
Qu, M., Xiong, L., Lyu, Y., Zhang, X., Shen, J., Guan, J., Chai, P., Lin, Z., Nie, B., Li, C., Xu, J., & Deng, H. (2021). Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration. Cell Research, 1–13. https://doi.org/10.1038/s41422-020-00453-x Cite
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Inhibition of Gli2 Suppresses Tumorigenicity in Glioblastoma Stem Cells Derived from a De Novo Murine Brain Cancer Model

The Sleeping-Beauty transposon-induced glioblastoma model was used in leucine-rich repeat-containing G-protein coupled receptor 5-GFP knock-in mice identify GFP-positive cells in neurosphere cultures from mouse glioblastoma tissues
[Cancer Gene Therapy]
Tanigawa, S., Fujita, M., Moyama, C., Ando, S., Ii, H., Kojima, Y., Fujishita, T., Aoki, M., Takeuchi, H., Yamanaka, T., Takahashi, Y., Hashimoto, N., & Nakata, S. (2021). Inhibition of Gli2 suppresses tumorigenicity in glioblastoma stem cells derived from a de novo murine brain cancer model. Cancer Gene Therapy, 1–14. https://doi.org/10.1038/s41417-020-00282-5 Cite
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The Epigenetic Regulator Mll1 Is Required for Wnt-Driven Intestinal Tumorigenesis and Cancer Stemness

Investigators identified the histone methyltransferase Mll1 as a regulator of Wnt-driven intestinal cancer. Mll1 is highly expressed in Lgr5+ stem cells and human colon carcinomas with increased nuclear β-catenin.
[Nature Communications]
Grinat, J., Heuberger, J., Vidal, R. O., Goveas, N., Kosel, F., Berenguer-Llergo, A., Kranz, A., Wulf-Goldenberg, A., Behrens, D., Melcher, B., Sauer, S., Vieth, M., Batlle, E., Stewart, A. F., & Birchmeier, W. (2020). The epigenetic regulator Mll1 is required for Wnt-driven intestinal tumorigenesis and cancer stemness. Nature Communications, 11(1), 6422. https://doi.org/10.1038/s41467-020-20222-z Cite
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Non-Canonical Wnt/PCP Signaling Regulates Intestinal Stem Cell Lineage Priming towards Enteroendocrine and Paneth Cell Fates

Scientists discovered non-canonical Wnt/planar cell polarity-activated intestinal stem cells that are primed towards the enteroendocrine or Paneth cell lineage.
[Nature Cell Biology]
Böttcher, A., Büttner, M., Tritschler, S., Sterr, M., Aliluev, A., Oppenländer, L., Burtscher, I., Sass, S., Irmler, M., Beckers, J., Ziegenhain, C., Enard, W., Schamberger, A. C., Verhamme, F. M., Eickelberg, O., Theis, F. J., & Lickert, H. (2021). Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates. Nature Cell Biology, 1–9. https://doi.org/10.1038/s41556-020-00617-2 Cite
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Lgr5 Marks Adult Progenitor Cells Contributing to Skeletal Muscle Regeneration and Sarcoma Formation

Researchers found that Lgr5, a receptor for Rspo and a potent mediator of Wnt/β-catenin signaling, marks a subset of activated satellite cells that contribute to muscle regeneration.
[Cell Reports]
Leung, C., Murad, K. B. A., Tan, A. L. T., Yada, S., Sagiraju, S., Bode, P. K., & Barker, N. (2020). Lgr5 Marks Adult Progenitor Cells Contributing to Skeletal Muscle Regeneration and Sarcoma Formation. Cell Reports, 33(12). https://doi.org/10.1016/j.celrep.2020.108535 Cite
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Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn’s Disease

Researchers drew parallels between the transcriptomes of ex vivo tissues and in vitro fetal organoids, revealing the maturation of organoid cultures in a dish.
[Developmental Cell]
Elmentaite, R., Ross, A. D. B., Roberts, K., James, K. R., Ortmann, D., Gomes, T., Nayak, K., Tuck, L., Pritchard, S., Bayraktar, O. A., Heuschkel, R., Vallier, L., Teichmann, S. A., & Zilbauer, M. (2020). Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn’s Disease. Developmental Cell. https://doi.org/10.1016/j.devcel.2020.11.010 Cite
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The Hippo–YAP Signaling as Guardian in the Pool of Intestinal Stem Cells

Scientists summarize recent advances in understanding the correlation between Hippo–YAP signaling and intestinal homeostasis, repair, and tumorigenesis, focusing specifically on intestinal stem cell regulation.
[Biomedicines]
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Unlike LGR4, LGR5 Potentiates Wnt–β-Catenin Signaling without Sequestering E3 Ligases

Scientists examined the mechanisms of action of LGR4 and LGR5 in parallel using coimmunoprecipitation, proximity ligation, competition binding, and time-resolved FRET assays in whole cells.
[Science Signaling]
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The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis

Colorectal cancer (CRC) tumoroids and a mouse model of CRC hepatic metastasis were used to test approaches to modify bone morphogenetic protein signaling and treat CRC.
[Gastroenterology]
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Unveiling Role of Sphingosine-1-Phosphate Receptor 2 as a Brake of Epithelial Stem Cell Proliferation and a Tumor Suppressor in Colorectal Cancer

Researchers mimicked the ablation of S1PR2 in normal mucosa by treating S1PR2+/+ organoids with JTE013 and characterized intestinal epithelial stem cells isolated from S1PR2-/-Lgr5-EGFP- mice.
[Journal of Experimental & Clinical Cancer Research]
Petti, L., Rizzo, G., Rubbino, F., Elangovan, S., Colombo, P., Restelli, S., Piontini, A., Arena, V., Carvello, M., Romano, B., Cavalleri, T., Anselmo, A., Ungaro, F., D’Alessio, S., Spinelli, A., Stifter, S., Grizzi, F., Sgambato, A., Danese, S., … Vetrano, S. (2020). Unveiling role of sphingosine-1-phosphate receptor 2 as a brake of epithelial stem cell proliferation and a tumor suppressor in colorectal cancer. Journal of Experimental & Clinical Cancer Research, 39(1), 253. https://doi.org/10.1186/s13046-020-01740-6 Cite
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