Disruption of the Crypt Niche Promotes Outgrowth of Mutated Colorectal Tumor Stem Cells

While azoxymethane delivery in vivo under non-adenoma-forming conditions yielded phenotypically normal mucosa and organoids derived thereof, niche injury ex vivo by progressive dextran sulfate sodium dose escalation facilitated outgrowth of Wnt-independent dysplastic organoids.