Pretreatment of Aged Mice with Retinoic Acid Supports Alveolar Regeneration via Upregulation of Reciprocal PDGFA Signaling

In vitro organoids generated from epithelial cells of mouse or patient with idiopathic pulmonary fibrosis co-cultured with young, aged or retinoic acid-pretreated murine fibroblasts were used to test potential targets.
[Thorax]
Gokey, J. J., Snowball, J., Green, J., Waltamath, M., Spinney, J. J., Black, K. E., Hariri, L. P., Xu, Y., & Perl, A. K. (2021). Pretreatment of aged mice with retinoic acid supports alveolar regeneration via upregulation of reciprocal PDGFA signalling. Thorax. https://doi.org/10.1136/thoraxjnl-2020-214986 Cite
AbstractFull Article
Bookmark

No account yet? Register

0
Share

Biomaterial-Guided Stem Cell Organoid Engineering for Modeling Development and Diseases

The authors discuss the enhancement of organoid systems for therapeutic applications using biofabrication and organoid-on-chip platforms, which facilitate the assembly of complex organoid systems for in vitro modeling of development and diseases.
[Acta Biomaterialia]
Hoang, P., & Ma, Z. (2021). Biomaterial-guided stem cell organoid engineering for modeling development and diseases. Acta Biomaterialia. https://doi.org/10.1016/j.actbio.2021.01.026 Cite
AbstractGraphical Abstract
Bookmark

No account yet? Register

0
Share

Epithelium-Derived Indian Hedgehog Restricts Stromal Expression of ErbB Family Members That Drive Colonic Tumor Cell Proliferation

Scientists found that recombinant EREG, BTC, and NRG1 but not Lgr5 ligand R-Spondin promoted growth and proliferation of Apc double mutant colonic organoids.
[Oncogene]
Westendorp, F., Karpus, O. N., Koelink, P. J., Vermeulen, J. L. M., Meisner, S., Koster, J., Büller, N. V. J. A., Wildenberg, M. E., Muncan, V., & van den Brink, G. R. (2021). Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation. Oncogene, 1–16. https://doi.org/10.1038/s41388-020-01633-0 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Re-Expression of REG Family and DUOXs Genes in CRC Organoids by Co-Culturing With CAFs

Researchers found that the expression levels of several genes, which were highly expressed in original colorectal carcinoma tissues, were downregulated in organoids but re-expressed in organoids by co-culturing with cancer-associated fibroblasts.
[Scientific Reports]
Naruse, M., Ochiai, M., Sekine, S., Taniguchi, H., Yoshida, T., Ichikawa, H., Sakamoto, H., Kubo, T., Matsumoto, K., Ochiai, A., & Imai, T. (2021). Re-expression of REG family and DUOX s genes in CRC organoids by co-culturing with CAFs. Scientific Reports, 11(1), 2077. https://doi.org/10.1038/s41598-021-81475-2 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Ex Vivo Culture of Intact Human Patient Derived Pancreatic Tumor Tissue

To fast-track translation of therapies and to inform personalised medicine, researchers aimed to develop a whole-tissue ex vivo explant model that maintains viability, 3D multicellular architecture, and microenvironmental cues of human pancreatic tumours.
[Scientific Reports]
Kokkinos, J., Sharbeen, G., Haghighi, K. S., Ignacio, R. M. C., Kopecky, C., Gonzales-Aloy, E., Youkhana, J., Timpson, P., Pereira, B. A., Ritchie, S., Pandzic, E., Boyer, C., Davis, T. P., Butler, L. M., Goldstein, D., McCarroll, J. A., & Phillips, P. A. (2021). Ex vivo culture of intact human patient derived pancreatic tumour tissue. Scientific Reports, 11(1), 1944. https://doi.org/10.1038/s41598-021-81299-0 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Advances in CNS Organoids: A Focus on Organoid-Based Models for Motor Neuron Disease

Scientists review recent advances in the development of CNS organoid models, their application in neurodegenerative diseases and technical limitations.
[Tissue Engineering Part C: Methods]
Vieira de Sa, R., Canizares Luna, M., & Pasterkamp, J. (2021). Advances in CNS organoids: a focus on organoid-based models for motor neuron disease. Tissue Engineering Part C: Methods. https://doi.org/10.1089/ten.TEC.2020.0337 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Greater Epithelial Ridge Cells Are the Principal Organoid-Forming Progenitors of the Mouse Cochlea

Scientists purified different cochlear cell types from neonatal mice, validated the composition of the different groups with single-cell RNA sequencing, and assessed the various groups’ potential to grow into inner ear organoids.
[Cell Reports]
Kubota, M., Scheibinger, M., Jan, T. A., & Heller, S. (2021). Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. Cell Reports, 34(3). https://doi.org/10.1016/j.celrep.2020.108646 Cite
Full ArticleGraphical Abstract
Bookmark

No account yet? Register

0
Share

Roles of TGF-β Superfamily Proteins in Extravillous Trophoblast Invasion

The authors detail recent advances in our understanding of the functions of TGF-β superfamily members and their corresponding receptors, signaling pathways, and downstream molecular targets in regulating human extravillous trophoblasts invasion from studies using various in vitro or ex vivo experimental models.
[Trends in Endocrinology and Metabolism]
Li, Y., Yan, J., Chang, H.-M., Chen, Z.-J., & Leung, P. C. K. (2021). Roles of TGF-β Superfamily Proteins in Extravillous Trophoblast Invasion. Trends in Endocrinology & Metabolism, 0(0). https://doi.org/10.1016/j.tem.2020.12.005 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Opposing Wnt Signals Regulate Cervical Squamocolumnar Homeostasis and Emergence of Metaplasia

Mouse lineage tracing, organoid culture and single-molecule RNA in situ hybridizations revealed that the two epithelia derive from separate cervix-resident lineage-specific stem cell populations regulated by opposing Wnt signals from the stroma.
[Nature Cell Biology]
Chumduri, C., Gurumurthy, R. K., Berger, H., Dietrich, O., Kumar, N., Koster, S., Brinkmann, V., Hoffmann, K., Drabkina, M., Arampatzi, P., Son, D., Klemm, U., Mollenkopf, H.-J., Herbst, H., Mangler, M., Vogel, J., Saliba, A.-E., & Meyer, T. F. (2021). Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia. Nature Cell Biology, 1–14. https://doi.org/10.1038/s41556-020-00619-0 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Proteomics Analysis of Human Intestinal Organoids during Hypoxia and Reoxygenation as a Model to Study Ischemia-Reperfusion Injury

Investigators demonstrated the use of human small intestinal organoids to model ischemia-reperfusion injury by exposing organoids to hypoxia and reoxygenation.
[Cell Death & Disease]
Kip, A. M., Soons, Z., Mohren, R., Duivenvoorden, A. A. M., Röth, A. A. J., Cillero-Pastor, B., Neumann, U. P., Dejong, C. H. C., Heeren, R. M. A., Olde Damink, S. W. M., & Lenaerts, K. (2021). Proteomics analysis of human intestinal organoids during hypoxia and reoxygenation as a model to study ischemia-reperfusion injury. Cell Death & Disease, 12(1), 1–13. https://doi.org/10.1038/s41419-020-03379-9 Cite
Full Article
Bookmark

No account yet? Register

0
Share

A CRISPR/Cas9-Engineered ARID1A-Deficient Human Gastric Cancer Organoid Model Reveals Essential and Non-Essential Modes of Oncogenic Transformation

CRISPR/Cas9-mediated ARID1A knockout in primary TP53-/- human gastric organoids induced morphologic dysplasia, tumorigenicity and mucinous differentiation.
[Cancer Discovery]
Lo, Y.-H., Kolahi, K. S., Du, Y., Chang, C.-Y., Krokhotin, A., Nair, A., Sobba, W. D., Karlsson, K., Jones, S. J., Longacre, T. A., Mah, A. T., Tercan, B., Sockell, A., Xu, H., Seoane, J. A., Chen, J., Shmulevich, I., Weissman, J. S., Curtis, C., … Kuo, C. J. (2021). A CRISPR/Cas9-engineered ARID1A-deficient human gastric cancer organoid model reveals essential and non-essential modes of oncogenic transformation. Cancer Discovery. https://doi.org/10.1158/2159-8290.CD-20-1109 Cite
Abstract
Bookmark

No account yet? Register

0
Share
Share