Microfabricated Disk Technology: Rapid Scale Up in Midbrain Organoid Generation

Researchers developed a method with microfabricated disks to scale up the generation of human midbrain organoids.
[Methods]
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ELAVL4, Splicing, and Glutamatergic Dysfunction Precede Neuron Loss in MAPT Mutation Cerebral Organoids

Scientists used human induced pluripotent stem cell-derived cerebral organoids expressing tau-V337M and isogenic corrected controls to discover early alterations because of the mutation that precede neurodegeneration.
[Cell]
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Single-Cell Transcriptomics Reveals Immune Response of Intestinal Cell Types to Viral Infection

Investigators developed a framework combining single-cell RNA-Seq and highly multiplex RNA FISH and applied it to human intestinal organoids infected with human astrovirus, a model human enteric virus.
[EMBO Reports]
Single-cell transcriptomics reveals immune response of intestinal cell types to viral infection. (2021). Molecular Systems Biology, 17(7), e9833. https://doi.org/10.15252/msb.20209833 Cite
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A Pan-Cancer Organoid Platform for Precision Medicine

Scientists described a robust pan-cancer tumor organoids platform with chemically defined media optimized on cultures acquired from over 1,000 patients.
[Cell Reports]
Larsen, B. M., Kannan, M., Langer, L. F., Leibowitz, B. D., Bentaieb, A., Cancino, A., Dolgalev, I., Drummond, B. E., Dry, J. R., Ho, C.-S., Khullar, G., Krantz, B. A., Mapes, B., McKinnon, K. E., Metti, J., Perera, J. F., Rand, T. A., Sanchez-Freire, V., Shaxted, J. M., … Salahudeen, A. A. (2021). A pan-cancer organoid platform for precision medicine. Cell Reports, 36(4). https://doi.org/10.1016/j.celrep.2021.109429 Cite
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Retinoblastoma from Human Stem Cell-Derived Retinal Organoids

To develop a laboratory model of human retinoblastoma formation, the authors made iPSCs from 15 participants with germline RB1 mutations. Each of the stem cell lines was validated, characterized and then differentiated into retina using a 3D organoid culture system.
[Nature Communications]
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SARS-CoV-2 Research Using Human Pluripotent Stem Cells and Organoids

Scientists summarize the latest results and issues of SARS-CoV-2 research using human ESC/iPSC-derived somatic cells and organoids.
[Stem Cells Translational Medicine]
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Blood Vessel Organoids Research from Penninger Lab to Play Central Role in 100 Years of Insulin UK-Canada Team Grant Research

LSI Director Dr. Josef Penninger and Drs. David Long, Luigi Gnudi, and Karen Price in the UK are recipients of a UK-Canada Diabetes Research Team Grant entitled “Bridging the gap to translation by understanding and preventing diabetic vascular complications using human organoid culture” as part of the 100 Years of Insulin initiative.
[The University of British Columbia]
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ELAVL4, Splicing, and Glutamatergic Dysfunction Precede Neuron Loss in MAPT Mutation Cerebral Organoids

Scientists used human induced pluripotent stem cell-derived cerebral organoids expressing tau-V337M and isogenic corrected controls to discover early alterations because of the mutation that precede neurodegeneration.
[Cell]
Bowles, K. R., Silva, M. C., Whitney, K., Bertucci, T., Berlind, J. E., Lai, J. D., Garza, J. C., Boles, N. C., Mahali, S., Strang, K. H., Marsh, J. A., Chen, C., Pugh, D. A., Liu, Y., Gordon, R. E., Goderie, S. K., Chowdhury, R., Lotz, S., Lane, K., … Temple, S. (2021). ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids. Cell, 0(0). https://doi.org/10.1016/j.cell.2021.07.003 Cite
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Development of a Quantitative Prediction Algorithm for Target Organ-Specific Similarity of Human Pluripotent Stem Cell-Derived Organoids and Cells

Researchers evaluated the similarity of generate human PSC-derived organoids and cells to human organs using a constructed quantitative calculation system.
[Nature Communications]
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Developing Human Pluripotent Stem Cell-Based Cerebral Organoids with a Controllable Microglia Ratio for Modeling Brain Development and Pathology

Investigators reported a new brain region-specific, microglia-containing organoid model by co-culturing human PSC-derived primitive neural progenitor cells and primitive macrophage progenitors.
[Stem Cell Reports]
Xu, R., Boreland, A. J., Li, X., Erickson, C., Jin, M., Atkins, C., Pang, Z. P., Daniels, B. P., & Jiang, P. (2021). Developing human pluripotent stem cell-based cerebral organoids with a controllable microglia ratio for modeling brain development and pathology. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2021.06.011 Cite
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Use of Human Tissue Stem Cell-Derived Organoid Cultures to Model Enterohepatic Circulation

Researchers demonstrated that mature stem cell-derived intestinal and liver organoid cultures each express functional molecules that modulate bile acid uptake and recycling.
[American Journal of Physiology-Gastrointestinal and Liver Physiology]
Blutt, S. E., Crawford, S., Bomidi, C., Zeng, X.-L., Broughman, J., Robertson, M., Coarfa, C., Tessier, M. E., Savidge, T. C., Hollinger, F. B., Curley, S., Donowitz, M., & Estes, M. K. (2021). Use of Human Tissue Stem Cell-Derived Organoid Cultures to Model Enterohepatic Circulation. American Journal of Physiology-Gastrointestinal and Liver Physiology. https://doi.org/10.1152/ajpgi.00177.2021 Cite
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