Scientists found that EPCAM + tumors developed in the mouse pancreas store zinc that is detectable by fluorescence-activated cell sorting using N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide (TSQ), a fluorescence chelator. EPCAM + TSQ + tumor cells isolated from the mouse pancreas formed organoids in matrigel.
[Biological Trace Element Research]
Retinal organoids were generated and organized into an in vitro assembloid model of the visual system with cortical and thalamic organoids.
[Stem Cell Reports]
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Fligor, C. M., Lavekar, S. S., Harkin, J., Shields, P. K., VanderWall, K. B., Huang, K.-C., Gomes, C., & Meyer, J. S. (2021). Extension of retinofugal projections in an assembled model of human pluripotent stem cell-derived organoids. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2021.05.009 Cite
A library of 17 C1-single and C1/C20-double modified salinomycin analogs was screened to identify compounds with improved activity against breast cancer stem cells.
[Biomedicine & Pharmacotherapy]
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Urbaniak, A., Reed, M. R., Fil, D., Moorjani, A., Heflin, S., Antoszczak, M., Sulik, M., Huczyński, A., Kupsik, M., Eoff, R. L., MacNicol, M. C., Chambers, T. C., & MacNicol, A. M. (2021). Single and double modified salinomycin analogs target stem-like cells in 2D and 3D breast cancer models. Biomedicine & Pharmacotherapy, 141, 111815. https://doi.org/10.1016/j.biopha.2021.111815 Cite
By targeting antiapoptotic proteins with specific BH3 mimetics in organoid models of colorectal cancer progression, scientists found that BCL-2 was essential only during instestinal stem cell transformation while MCL1 inhibition did not affect adenoma outgrowth.
[Cell Death & Differentiation]
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The authors isolated basal cells from the rat epididymis and established 3-dimensional cell cultures from the basal and non-basal cell fractions. Organoids were formed by single adult epididymal basal cells.
[Biology of Reproduction]
Multi-cellular hepatocellular carcinoma (HCC) organoid models were established which contained various types of stromal cells, such as hepatic stellate cells, fibroblasts, and endothelial cells together with HCC cells.
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Upon treatment with N,N,N′,N′-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine, a zinc chelator, organoids degenerated and its negative effect was rescued by co-treatment with zinc, indicating that zinc is necessary for the growth and survival of tumor organoids.
[Biological Trace Element Research]
Scientists reported the generation of a hepatobiliary tubular organoid using mouse hepatocyte progenitors and cholangiocytes.
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Tanimizu, N., Ichinohe, N., Sasaki, Y., Itoh, T., Sudo, R., Yamaguchi, T., Katsuda, T., Ninomiya, T., Tokino, T., Ochiya, T., Miyajima, A., & Mitaka, T. (2021). Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo. Nature Communications, 12(1), 3390. https://doi.org/10.1038/s41467-021-23575-1 Cite
Investigators studied effects of recombinant D-dopachrome tautomerase (DDT) on cell proliferation and survival by clonogenic assay and annexin V-PI staining respectively. DDT-induced signaling was investigated by Western blot.
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Song, S., Liu, B., Habibie, H., Bor, J. van den, Smit, M. J., Gosens, R., Wu, X., Brandsma, C.-A., Cool, R. H., Haisma, H. J., Poelarends, G. J., & Melgert, B. N. (2021). D-dopachrome tautomerase contributes to lung epithelial repair via atypical chemokine receptor 3-dependent Akt signaling. EBioMedicine, 68. https://doi.org/10.1016/j.ebiom.2021.103412 Cite
The molecular networks that activate in cells in response to mechanical stimuli are yet not well-understood. Our limited knowledge stems partially from the lack of available tools that are capable of exerting controlled mechanical stress to individual cells and at the same time observing their responses at subcellular to molecular resolution.
[Journal of Applied Physics]
An official opening ceremony for the Hub will take place once it is permissible to do so. For the first Hub program – research into organoid models – Dr. Kai Wicker and his team have moved into the EKFZ for Digital Health on the campus of the Carl Gustav Carus University Hospital – a stone’s throw away from top experts in the field.
[Technische Universität Dresden (EurekAlert!)]
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