Forskolin-Induced Organoid Swelling Is Associated with Long-Term CF Disease Progression

Individual cystic fibrosis transmembrane conductance regulator function was defined by FIS, measured as the relative size increase of intestinal organoids after stimulation with 0.8 µM forskolin, quantified as area under the curve.
[European Respiratory Journal]
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Kidney Organoids Recapitulate Human Basement Membrane Assembly in Health and Disease

Researchers investigated the dynamics of basement membranes assembly in human pluripotent stem cell-derived kidney organoids.
[eLife]
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Identification of Shared Tumor Epitopes from Endogenous Retroviruses Inducing High-Avidity Cytotoxic T Cells for Cancer Immunotherapy

Six candidates among the most commonly shared HLA-A2 epitopes with evidence of translation were selected for immunological evaluation. In vitro priming assays confirmed the immunogenicity of these epitopes, which induced high-avidity CD8+ T cell clones.
[Science Advances]
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A Simple Method to Generate Human Airway Epithelial Organoids with Externally-Oriented Apical Membranes

Investigators described a simple method to generate organoid cultures using isolated human tracheobronchial epithelial cells grown in mixed matrix components and supplemented at day 14 with the Wnt pathway agonist R-spondin 2 and the bone morphogenic protein antagonist Noggin.
[American Journal of Physiology-Lung Cellular and Molecular Physiology]
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Biocompatible Micro Tweezers for 3D Hydrogel Organoid Array Mechanical Characterization

Researchers presented novel biocompatible Polydimethylsiloxane-based micromechanical tweezers capable of the stiffness characterization and manipulation of hydrogel-based organoids.
[PLoS One]
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Modeling Human Thyroid Development by Fetal Tissue-Derived Organoid Culture

Investigators established a long-term culture of human fetal thyroid organoids system, which retained the fetal thyroid lineages and molecular signatures, as well as the ability to generate functional human thyroid follicles post mice renal transplantation.
[Advanced Science]
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Recapitulating Lipid Accumulation and Related Metabolic Dysregulation in Human Liver-Derived Organoids

Scientists developed an organoid-based 3D culture model to recapitulate key features of fatty liver disease focusing on intracellular lipid accumulation and metabolic dysregulation.
[Journal of Molecular Medicine]
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Biomimetic Models of the Glomerulus

Scaffold-based 3D culture systems and organoids provide greater microenvironmental complexity but do not replicate fluid flows and dynamic responses to fluidic stimuli.
[Nature Reviews Nephrology]
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Single-Cell ATAC-Seq of Fetal Human Retina and Stem-Cell-Derived Retinal Organoids Shows Changing Chromatin Landscapes during Cell Fate Acquisition

Researchers extended the transcriptomic analyses to incorporate single-cell assay for transposase-accessible chromatin sequencing, a powerful method used to characterize potential gene regulatory networks through the changes in accessible chromatin that accompany cell-state changes.
[Cell Reports]
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Fasting Improves Therapeutic Response in Hepatocellular Carcinoma through p53-Dependent Metabolic Synergism

Scientists showed in hepatocellular carcinoma (HCC) cells, xenografts, and patient-derived organoids that fasting improved sorafenib efficacy and acted synergistically to sensitize sorafenib-resistant HCC.
[Science Advances]
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SARS-CoV-2 Infection Triggers Paracrine Senescence and Leads to a Sustained Senescence-Associated Inflammatory Response

In cultured human cells or bronchial organoids, SASR-CoV-2 infection-induced senescent cells expressed high levels of a series of inflammatory factors known as senescence-associated secretory phenotypes 4 in a sustained manner, even after SARS-CoV-2 was no longer detectable.
[Nature Aging]
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