Tag Archives: cell culture

Genetic Manipulation and Immortalized Culture of Ex Vivo Primary Human Germinal Center B Cells

Researchers describe a detailed protocol for the purification and ex vivo expansion of primary, nonmalignant human germinal center B cells.
[Nature Protocols]
Caeser, R., Gao, J., Di Re, M., Gong, C., & Hodson, D. J. (2021). Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells. Nature Protocols, 1–21. https://doi.org/10.1038/s41596-021-00506-4 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Human Airway Cells Prevent SARS-CoV-2 Multibasic Cleavage Site Cell Culture Adaptation

Researchers report that propagating SARS-CoV-2 on the human airway cell line Calu-3 prevented cell culture adaptations in the multibasic cleavage sites. Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation.
[eLife]
Lamers, M. M., Mykytyn, A. Z., Breugem, T. I., Wang, Y., Wu, D. C., Riesebosch, S., van den Doel, P. B., Schipper, D., Bestebroer, T., Wu, N. C., & Haagmans, B. L. (2021). Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation. ELife, 10, e66815. https://doi.org/10.7554/eLife.66815 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Global Changes to HepG2 Cell Metabolism in Response to Galactose Treatment

Scientists performed transcriptomic and metabolomic analyses on human hepatocellular carcinoma cells adapted to either glucose or galactose as the aldohexose source.
[American Journal of Physiology-Cell Physiology]
Full ArticlePress Release
Bookmark

No account yet? Register

0
Share

The Mechanistic Basis of Protection by Non-neutralizing Anti-Alphavirus Antibodies

Scientists evaluated the activity of 13 non-neutralizing monoclonal antibodies against Mayaro virus, an emerging arthritogenic alphavirus.
[Cell Reports]
Earnest, J. T., Holmes, A. C., Basore, K., Mack, M., Fremont, D. H., & Diamond, M. S. (2021). The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell Reports, 35(1). https://doi.org/10.1016/j.celrep.2021.108962 Cite
Full ArticleGraphical Abstract
Bookmark

No account yet? Register

0
Share

Incense Smoke-Induced Oxidative Stress Disrupts Tight Junctions and Bronchial Epithelial Barrier Integrity and Induces Airway Hyperresponsiveness in Mouse Lungs

Using mouse and cell culture models, the authors evaluated the effects of incense smoke exposure on airway hyperresponsiveness, expression of multiple epithelial tight junction- and adherens junction-associated mRNAs and proteins in the lungs, and the barrier function of bronchial epithelial cells assessed by transepithelial electronic resistance.
[Scientific Reports]
Full Article
Bookmark

No account yet? Register

0
Share

Disparate Temperature-Dependent Virus–Host Dynamics for SARS-CoV-2 and SARS-CoV in the Human Respiratory Epithelium

Since different temperatures encountered in the human upper and lower respiratory tract have been shown to affect the replication kinetics of several respiratory viruses, as well as host immune response dynamics, scientists investigated the impact of temperatures during SARS-CoV-2 and SARS-CoV infection using the primary human airway epithelial cell culture model.
[PLoS Biology]
Full Article
Bookmark

No account yet? Register

0
Share

A Microfabricated Multi-Compartment Device for Neuron and Schwann Cell Differentiation

Scientists report a multi-compartment microfluidic device where up to three different cell populations could be cultured in a fluidically independent circuit. The device allows cell migration across the compartments and their differentiation.
[Scientific Reports]
De Vitis, E., La Pesa, V., Gervaso, F., Romano, A., Quattrini, A., Gigli, G., Moroni, L., & Polini, A. (2021). A microfabricated multi-compartment device for neuron and Schwann cell differentiation. Scientific Reports, 11(1), 7019. https://doi.org/10.1038/s41598-021-86300-4 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Two Sequential Activation Modules Control the Differentiation of Protective T Helper-1 (Th1) Cells

Scientists assessed Th1 cell generation in vivo by studying antigen-specific CD4+ T cells during infection with the phagosomal pathogen Salmonella enterica, or influenza A virus, for which CD4+ T cells were less important.
[Immunity]
Krueger, P. D., Goldberg, M. F., Hong, S.-W., Osum, K. C., Langlois, R. A., Kotov, D. I., Dileepan, T., & Jenkins, M. K. (2021). Two sequential activation modules control the differentiation of protective T helper-1 (Th1) cells. Immunity, 0(0). https://doi.org/10.1016/j.immuni.2021.03.006 Cite
Abstract
Bookmark

No account yet? Register

0
Share

A Thermo-Responsive Collagen-Nanocellulose Hydrogel for the Growth of Intestinal Organoids

Scientists investigated collagen-nanocellulose hydrogels as a thermo-responsive matrix for the formation and growth of intestinal organoids.
[Materials Science and Engineering: C]
Curvello, R., Alves, D., Abud, H. E., & Garnier, G. (2021). A thermo-responsive collagen-nanocellulose hydrogel for the growth of intestinal organoids. Materials Science and Engineering: C, 112051. https://doi.org/10.1016/j.msec.2021.112051 Cite
AbstractGraphical Abstract
Bookmark

No account yet? Register

0
Share

Isolating and Targeting the Real-Time Plasticity and Malignant Properties of Epithelial-Mesenchymal Transition in Cancer

3D high-content screening of ~23,000 compounds using dual-reporter mesenchymal SW620 tumor organoids identified small molecule probes that modulate EMT, and a subset of probes that effectively induced MET.
[Oncogene]
Esquer, H., Zhou, Q., Nemkov, T., Abraham, A. D., Rinaldetti, S., Chen, Y.-C., Zhang, X., Orman, M. V., D’Alessandro, A., Ferrer, M., Messersmith, W. A., & LaBarbera, D. V. (2021). Isolating and targeting the real-time plasticity and malignant properties of epithelial-mesenchymal transition in cancer. Oncogene, 1–14. https://doi.org/10.1038/s41388-021-01728-2 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Identification of Extracellular Matrix Proteins Secreted by Human Dermal Fibroblasts Cultured in 3D Electrospun Scaffolds

Researchers constructed an artificial 3D tumor by culturing human breast cancer JIMT-1 cells and human dermal fibroblasts in a 3D network of electrospun polycaprolactone fibres. They investigated ECM components produced by the cells in the artificial 3D tumor, which is an important step in validating the model.
[Scientific Reports]
Malakpour-Permlid, A., Buzzi, I., Hegardt, C., Johansson, F., & Oredsson, S. (2021). Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds. Scientific Reports, 11(1), 6655. https://doi.org/10.1038/s41598-021-85742-0 Cite
Full Article
Bookmark

No account yet? Register

0
Share
Share