The Histone H2B Ubiquitin Ligase RNF40 Is Required for HER2-Driven Mammary Tumorigenesis

Scientists utilized human tumor samples, cell culture models, and a mammary carcinoma mouse model with tissue-specific Rnf40 deletion and identified an unexpected tumor-supportive role of RNF40 in HER2-positive breast cancer subtype.
[Cell Death & Disease]
Wegwitz, F., Prokakis, E., Pejkovska, A., Kosinsky, R. L., Glatzel, M., Pantel, K., Wikman, H., & Johnsen, S. A. (2020). The histone H2B ubiquitin ligase RNF40 is required for HER2-driven mammary tumorigenesis. Cell Death & Disease, 11(10), 1–14. https://doi.org/10.1038/s41419-020-03081-w Cite
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Automated Microfluidic Platform for Dynamic and Combinatorial Drug Screening of Tumor Organoids

Scientists present an automated, high-throughput, microfluidic 3D organoid culture and analysis system to facilitate preclinical research and personalized therapies. They validated the system by performing individual, combinatorial, and sequential drug screens on human-derived pancreatic tumor organoids.
[Nature Communications]
Schuster, B., Junkin, M., Kashaf, S. S., Romero-Calvo, I., Kirby, K., Matthews, J., Weber, C. R., Rzhetsky, A., White, K. P., & Tay, S. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11(1), 5271. https://doi.org/10.1038/s41467-020-19058-4 Cite
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Molecular Biological Analysis of 5-FU-Resistant Gastric Cancer Organoids; KHDRBS3 Contributes to the Attainment of Features of Cancer Stem Cell

Scientists established GC organoids (GCOs) and gradually treated them with higher concentrations of 5-FU. They successfully harvested four 5-FU-resistant GCOs, which were supported by significant changes in the expression of molecules related to 5-FU metabolism. Microarray analysis was peformed using three normal gastric organoids and three pairs of 5-FU-resistant and parental GCOs.
[Oncogene]
Ukai, S., Honma, R., Sakamoto, N., Yamamoto, Y., Pham, Q. T., Harada, K., Takashima, T., Taniyama, D., Asai, R., Fukada, K., Naka, K., Tanabe, K., Ohdan, H., & Yasui, W. (2020). Molecular biological analysis of 5-FU-resistant gastric cancer organoids; KHDRBS3 contributes to the attainment of features of cancer stem cell. Oncogene, 1–14. https://doi.org/10.1038/s41388-020-01492-9 Cite
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Axol Biosciences Offers New Screening Assay for hiPSC-Derived Cells

Axol Biosciences has added multi-electrode array (MEA) screening services for human-iPSC-derived cells to its service offering for preclinical drug discovery. The company said that its new MEA screening services optimize hiPSC-derived cell culture, while also providing electrophysiology acquisition and analysis from physiologically relevant human cell models.
[The Science Advisory Board]
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Cystatin C Regulates the Cytotoxicity of Infection‐Induced Endothelial‐Derived β‐Amyloid

Mass spectrometry of endothelial cell culture media following P. aeruginosa infection allowed identification of multiple potential secreted modulators of beta amyloid, including cystatin C, gelsolin, and ApoJ/clusterin.
[Febs Open Bio]
Balczon, R., Morrow, K. A., Leavesley, S., Francis, C. M., Stevens, T. C., Agwaramgbo, E., Williams, C., Stevens, R. P., Langham, G., Voth, S., Cioffi, E. A., Weintraub, S. E., & Stevens, T. (n.d.). Cystatin C regulates the cytotoxicity of infection-induced endothelial-derived β-amyloid. FEBS Open Bio, n/a(n/a). https://doi.org/10.1002/2211-5463.12997 Cite
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Extracellular Matrix-Bound FGF2 Mediates Estrogen Receptor Signaling and Therapeutic Response in Breast Cancer

Scientists uncovered that estrogen receptor-positive breast cancer cells cultured within extracellular matrix -scaffolds had an increase in ER signaling that occured via a MAPK-dependent, but estrogen-independent manner.
[Molecular Cancer Research]
DiGiacomo, J. W., Godet, I., Trautmann-Rodriguez, M., & Gilkes, D. M. (2020). Extracellular matrix-bound FGF2 mediates estrogen receptor signaling and therapeutic response in breast cancer. Molecular Cancer Research. https://doi.org/10.1158/1541-7786.MCR-20-0554 Cite
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Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors

Scientists highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography.
[Frontiers in Bioengineering and Biotechnology]
Tran, R., Moraes, C., & Hoesli, C. A. (2020). Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.583970 Cite
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Isolation and Culture of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells

Scientists present a protocol to isolate and culture brain cells from two-month human iPSC-derived cerebral organoids. The dissociation and plating of cells from organoids takes three to four hours.
[Methods in Molecular Biology]
Yan, Y., Arzua, T., Logan, S., & Bai, X. (n.d.). Isolation and Culture of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells (pp. 1–12). Springer US. https://doi.org/10.1007/7651_2020_328 Cite
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Effects of Fibrillin Mutations on the Behavior of Heart Muscle Cells in Marfan Syndrome

Both induced pluripotent stem cells derived from a Marfan syndrome (MFS)-patient and the line with the corrected fibrillin-1 mutation were differentiated to cardiomyocytes. Several functional analyses were performed on this model to study MFS related cardiomyopathy.
[Scientific Reports]
Aalders, J., Léger, L., Van der Meeren, L., Van den Vreken, N., Skirtach, A. G., Sinha, S., De Backer, J., & van Hengel, J. (2020). Effects of fibrillin mutations on the behavior of heart muscle cells in Marfan syndrome. Scientific Reports, 10(1), 16756. https://doi.org/10.1038/s41598-020-73802-w Cite
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Microtechnology-Based Methods for Organoid Models

The authors discuss the most recent production methods, challenges currently faced in organoid and spheroid production, and microfabricated and microfluidic applications for improving spheroid and organoid generation.
[Microsystems & Nanoengineering]
Velasco, V., Shariati, S. A., & Esfandyarpour, R. (2020). Microtechnology-based methods for organoid models. Microsystems & Nanoengineering, 6(1), 1–13. https://doi.org/10.1038/s41378-020-00185-3 Cite
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Staphylococcus aureus Second Immunoglobulin-Binding Protein Drives Atopic Dermatitis via IL-33

In vitro human keratinocyte cell culture, ex vivo human skin organ explants and the eczema prone Nc/Tnd mouse were used as model systems to assess type-2 promoting immune responses to S. aureus.
[Journal of Allergy and Clinical Immunology]
Kindi, A. A., Williams, H., Matsuda, K., Alkahtani, A. M., Saville, C., Bennett, H., Alshammari, Y., Tan, S. Y., O’Neill, C., Tanaka, A., Matsuda, H., Arkwright, P. D., & Pennock, J. L. (2020). Staphylococcus aureus Second Immunoglobulin-Binding Protein drives atopic dermatitis via IL-33. Journal of Allergy and Clinical Immunology, 0(0). https://doi.org/10.1016/j.jaci.2020.09.023 Cite
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