Researchers describe a detailed protocol for the purification and ex vivo expansion of primary, nonmalignant human germinal center B cells.
Researchers report that propagating SARS-CoV-2 on the human airway cell line Calu-3 prevented cell culture adaptations in the multibasic cleavage sites. Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation.
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Lamers, M. M., Mykytyn, A. Z., Breugem, T. I., Wang, Y., Wu, D. C., Riesebosch, S., van den Doel, P. B., Schipper, D., Bestebroer, T., Wu, N. C., & Haagmans, B. L. (2021). Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation. ELife, 10, e66815. https://doi.org/10.7554/eLife.66815 Cite
Scientists performed transcriptomic and metabolomic analyses on human hepatocellular carcinoma cells adapted to either glucose or galactose as the aldohexose source.
[American Journal of Physiology-Cell Physiology]
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Scientists evaluated the activity of 13 non-neutralizing monoclonal antibodies against Mayaro virus, an emerging arthritogenic alphavirus.
Using mouse and cell culture models, the authors evaluated the effects of incense smoke exposure on airway hyperresponsiveness, expression of multiple epithelial tight junction- and adherens junction-associated mRNAs and proteins in the lungs, and the barrier function of bronchial epithelial cells assessed by transepithelial electronic resistance.
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Since different temperatures encountered in the human upper and lower respiratory tract have been shown to affect the replication kinetics of several respiratory viruses, as well as host immune response dynamics, scientists investigated the impact of temperatures during SARS-CoV-2 and SARS-CoV infection using the primary human airway epithelial cell culture model.
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Scientists report a multi-compartment microfluidic device where up to three different cell populations could be cultured in a fluidically independent circuit. The device allows cell migration across the compartments and their differentiation.
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Scientists assessed Th1 cell generation in vivo by studying antigen-specific CD4+ T cells during infection with the phagosomal pathogen Salmonella enterica, or influenza A virus, for which CD4+ T cells were less important.
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Scientists investigated collagen-nanocellulose hydrogels as a thermo-responsive matrix for the formation and growth of intestinal organoids.
[Materials Science and Engineering: C]
3D high-content screening of ~23,000 compounds using dual-reporter mesenchymal SW620 tumor organoids identified small molecule probes that modulate EMT, and a subset of probes that effectively induced MET.
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Esquer, H., Zhou, Q., Nemkov, T., Abraham, A. D., Rinaldetti, S., Chen, Y.-C., Zhang, X., Orman, M. V., D’Alessandro, A., Ferrer, M., Messersmith, W. A., & LaBarbera, D. V. (2021). Isolating and targeting the real-time plasticity and malignant properties of epithelial-mesenchymal transition in cancer. Oncogene, 1–14. https://doi.org/10.1038/s41388-021-01728-2 Cite
Researchers constructed an artificial 3D tumor by culturing human breast cancer JIMT-1 cells and human dermal fibroblasts in a 3D network of electrospun polycaprolactone fibres. They investigated ECM components produced by the cells in the artificial 3D tumor, which is an important step in validating the model.
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