The authors discuss current understanding of the effects of different 3D cell culture systems on proliferation, viability and osteogenic differentiation, as well as on the immunomodulatory and anti-inflammatory potential of MSCs.
[Stem Cell Research & Therapy]
The authors demonstrated that Pannexin 3 (Panx3) regulated skin development by modulating the transcription factor, Epiprofin (Epfn). In cell culture, Panx3 overexpression promoted HaCaT cell differentiation, cell cycle exit and enhanced Epfn expression.
Investigators identified the role of retinal pigment epithelium (RPE) basement membrane proteins type IV collagen, laminin, and nidogen-1 in the maturation and functionality of humn ESC-RPE after cryopreservation.
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Viheriälä, T., Sorvari, J., Ihalainen, T. O., Mörö, A., Grönroos, P., Schlie-Wolter, S., Chichkov, B., Skottman, H., Nymark, S., & Ilmarinen, T. (2021). Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE. Scientific Reports, 11(1), 933. https://doi.org/10.1038/s41598-020-79638-8 Cite
Scientists present an in vitro model of the peripheral nervous system by establishing a coculture model of motor neurons and Schwann cells in a 3D environment in a microengineered extracellular matrix hydrogel scaffold.
[NPG Asia Materials]
The authors introduce its application in developing cancer preclinical models, detecting cancer biomarkers, screening anti-cancer drugs, exploring tumor heterogeneity and producing nano-drugs. They highlight the functions and recent development of microfluidic chip to provide references for advancing cancer diagnosis and treatment.
Scientists discuss the different cell types used to prepare heterocellular organoids for islet transplantation and their contribution to the organoids design.
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Recent advances in the design of implantable insulin secreting heterocellular islet organoids - ScienceDirect. (n.d.). Retrieved January 5, 2021, from https://www.sciencedirect.com/science/article/abs/pii/S0142961220308747 Cite
Cellulosic matrices were cross-linked with Ca2+ and Mg2+ and intestinal organoids cultured for four days. The organoids were recovered for passaging and RNA extraction. TEMPO-periodate-oxidized nanocellulose fibers formed functionalized hydrogels and supported the growth of intestinal organoids.
Researchers developed an alginate scaffold with human umbilical cord mesenchymal stem cell exosomes to treat nerve injury-induced pain. In vitro studies of PC12 and HEK293 cells were used to evaluate the neuroprotective and neurotrophic effects of exosomes.
[Journal of Pain Research]
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Hsu, J.-M., Shiue, S.-J., Yang, K. D., Shiue, H.-S., Hung, Y.-W., Pannuru, P., Poongodi, R., Lin, H.-Y., & Cheng, J.-K. (2020, December 4). <p>Locally Applied Stem Cell Exosome-Scaffold Attenuates Nerve Injury-Induced Pain in Rats</p>. Journal of Pain Research. https://doi.org/10.2147/JPR.S286771 Cite
Scientists showed that PKCδ is a secretory protein that regulated cell growth of liver cancer. Full-length PKCδ was secreted to the extracellular space in living liver cancer cells under normal cell culture conditions and in xenograft mouse models.
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Yamada, K., Oikawa, T., Kizawa, R., Motohashi, S., Yoshida, S., Kumamoto, T., Saeki, C., Nakagawa, C., Shimoyama, Y., Aoki, K., Tachibana, T., Saruta, M., Ono, M., & Yoshida, K. (2020). Unconventional secretion of PKCδ exerts tumorigenic function via stimulation of ERK1/2 signaling in liver cancer. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-20-2009 Cite
Researchers describe a modified protocol that can be used to reliably generate cerebral organoids of a telencephalic identity and maintain long-term viability for later stages of neural development, including axon outgrowth and neuronal maturation.
To determine the presence of CB1 and CB2 receptors in pancreatic β cells, immunofluorescence of primary cell cultures and pancreatic sections was performed.
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Barajas-Martínez, A., Bermeo, K., Cruz, L. de la, Martínez-Vargas, M., Martínez-Tapia, R. J., García, D. E., & Navarro, L. (2020). Cannabinoid receptors are differentially regulated in the pancreatic islets during the early development of metabolic syndrome. Islets, 0(0), 1–11. https://doi.org/10.1080/19382014.2020.1849927 Cite