RT‐qPCR was used to measure the expression of genes. Actinomycin D and RNase R were employed to examine the stability of circVAPA. CCK‐8, EdU, Transwell, sphere formation assay, and western blot analysis were conducted to examine the changes of NSCLC cells in response to circVAPA knockdown. Luciferase reporter assay was conducted for molecular mechanism.
[Journal of Gene Medicine]
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Researchers showed that the nuclear lamina protein lamin B1 (LB1) was downregulated with age in mouse hippocampal neural stem cells, whereas protein levels of SUN-domain containing protein 1, previously implicated in Hutchinson-Gilford progeria syndrome, increased.
[Cell Stem Cell]
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Researchers isolated exosomes derived from umbilical cord MSCs grown in a 3D culture system and explored their ability to modulate the photo-aging of HaCaT keratinocytes.
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Liu, S.-J., Meng, M.-Y., Han, S., Gao, H., Zhao, Y.-Y., Yang, Y., Lin, Z.-Y., Yang, L.-R., Zhu, K., Han, R., Huang, W.-W., Wang, R.-Q., Yang, L.-L., Wang, W.-J., Li, L., Wang, X.-D., Hou, Z.-L., Liao, L. W., & Yang, L. (2021). Umbilical cord mesenchymal stem cell-derived exosomes ameliorate HaCaT cell photo-aging. Rejuvenation Research. https://doi.org/10.1089/rej.2020.2313 Cite
Exosomes were isolated from MSCs using ultracentrifugation, and further characterized by the nanoparticle tracking analyzer and flow cytometry. RNA sequencing, Western Blot, in vitro cell proliferation, in vivo pro-angiogenesis, as well as ischemic repairment of diabetic foot through rat model were performed to evaluate exosome physiological functions.
[Stem Cell Research]
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Huang, C., Luo, W., Wang, Q., Ye, Y., Fan, J., Lin, L., Shi, C., Wei, W., Chen, H., Wu, Y., & Tang, Y. (2021). Human mesenchymal stem cells promote ischemic repairment and angiogenesis of diabetic foot through exosome miRNA-21-5p. Stem Cell Research, 52, 102235. https://doi.org/10.1016/j.scr.2021.102235 Cite
In vitro analysis demonstrated that myofibroblasts promoted cell proliferation, invasion, and epithelial‐mesenchymal transformation of hepatocellular carcionoma cells, whereas Musashi RNA binding protein 2 (Msi2) deletion in myofibroblasts reversed these effects.
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Qu, C., He, L., Yao, N., Li, J., Jiang, Y., Li, B., Peng, S., Hu, K., Chen, D., Chen, G., Huang, W., Cao, M., Fan, J., Yuan, Y., Ye, W., & Hong, J. (n.d.). Myofibroblast-specific Msi2 knockout inhibits hepatocellular carcinoma progression in a mouse model. Hepatology, n/a(n/a). https://doi.org/https://doi.org/10.1002/hep.31754 Cite
Interleukin (IL)‐33 was highly expressed in the skin and pancreatic epithelial cells in chronic inflammation, leading to a markedly repressed Smad6 expression as well as dramatically upregulated p‐SMAD2/3 and p‐SMAD1/5 in the epithelial cells.
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Nuclear IL‐33/SMAD signaling axis promotes cancer development in chronic inflammation | The EMBO Journal. (n.d.). Retrieved February 23, 2021, from https://www.embopress.org/doi/abs/10.15252/embj.2020106151 Cite
The BMP signaling pathway is widely regarded as one of the most important pathways in vertebrate skeletal biology, of which BMP2 is a potent inducer, governing the osteoblast differentiation of bone marrow stromal cells. Scientists used RNA-seq, ATAC-seq, and animal models to characterize the BMP2-dependent gene regulatory network governing osteoblast lineage commitment.
[Cell Death & Disease]
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Yu, S., Guo, J., Sun, Z., Lin, C., Tao, H., Zhang, Q., Cui, Y., Zuo, H., Lin, Y., Chen, S., Liu, H., & Chen, Z. (2021). BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation. Cell Death & Disease, 12(2), 1–17. https://doi.org/10.1038/s41419-021-03480-7 Cite
This guide on the composition and functions of the ECM gives a broad overview of the matrisome, the major ECM macromolecules and their interaction networks within the ECM and with the cell surface, summarizes their main structural features and their roles in tissue organization and cell functions, and emphasizes the importance of specific ECM constituents in disease development and progression as well as the advances in molecular targeting of ECM to design new therapeutic strategies.
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A Guide to the Composition and Functions of the Extracellular Matrix - Karamanos - - The FEBS Journal - Wiley Online Library. (n.d.). Retrieved February 23, 2021, from https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15776 Cite
Investigators showed that Z-DNA-binding protein 1 (ZBP1) was selectively expressed in late B cell development in both human and mouse cells and it is required for optimal T-cell-dependent humoral immune responses.
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Ponnusamy, K., Tzioni, M. M., Begum, M., Robinson, M. E., Caputo, V. S., Katsarou, A., Trasanidis, N., Xiao, X., Kostopoulos, I. V., Iskander, D., Roberts, I., Trivedi, P., Auner, H. W., Naresh, K., Chaidos, A., & Karadimitris, A. (2020). The innate sensor ZBP1-IRF3 axis regulates cell proliferation in multiple myeloma. Haematologica. https://doi.org/10.3324/haematol.2020.274480 Cite
Researchers demonstrated that SuFu Negating Protein 1 (SNEP1) acted as a novel feedback regulator of hedgehog (Hh) signaling by destabilizing SuFu and promoting tumor growth and anti-Hh resistance.
[Cell Death & Disease]
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Scientists discuss how organelles of intestinal cells serve to initiate, mediate, and terminate signals, that are vital for homeostasis.
[Trends in Cell Biology]