CRISPR-Cas9

[Nature Communications] Investigators utilized forebrain organoids generated from induced pluripotent stem cells of patients with a syndromic form of Autism Spectrum Disorder with a homozygous protein-truncating mutation in CNTNAP2, to study its effects on embryonic cortical development.

[Cancer Immunology Research] To identify epigenetic regulators of tumor antigen expression, scientists established a transplantable syngeneic tumor model of immune escape with silenced antigen expression and used this system as a platform for a CRISPR-Cas9 suppressor screen for genes encoding epigenetic modifiers.

[Life Sciences] Scientists investigated the relationship between melanin production and lipofuscin synthesis in normal mouse melanoma cell line B16F1 cells and Tyrosinase gene knockout cells.

[Cell Death & Disease] Scientists demonstrated that the prototypical Smac mimetic BV6 cooperated with the stimulator of interferon genes ligand 2′,3′-cyclic guanosine monophosphate–adenosine monophosphate to trigger necroptosis in apoptosis-deficient pancreatic cancer cells

[Cell Death & Disease] The exact role of SOX10 in human neural crest development and the underlying molecular mechanisms of SOX10-related human diseases remain poorly understood due to the lack of appropriate human model systems. Researchers successfully generated SOX10-knockout human induced pluripotent stem cells by the CRISPR-Cas9 gene editing tool.

[Scientific Reports] Scientists developed a homozygous NaV1.5 KO iPSC line able to differentiate into cardiomyocytes with CRISPR/Cas9 tool. The NaV1.5 KO iPSC-cardiomyocytes exhibited an organized contractile apparatus, spontaneous contractile activity, and electrophysiological recordings confirmed the major reduction in total Na+ currents.