CRISPR-Cas9

[Nature Communications] Researchers engineered gRNAs with highly stable hairpins in their constant parts and further enhanced their stability by chemical modifications.

[Cancer Research] Scientists generated models of sarcomas bearing kinase fusions and assessed their response to molecularly targeted therapy. Immortalized, untransformed human MSCs, a putative cell-of-origin of sarcomas, were modified using CRISPR-Cas9 to harbor a RET chromosomal translocation.

[Science Advances] The authors provided experimental evidence demonstrating a previously unidentified causal role of disturbed flow-induced endothelial thioredoxin domain containing 5 (TXNDC5) in atherosclerosis.

[Stem Cell Reports] Investigators discussed available tools, including CRISPR-Cas9, and the applications of these tools to develop cell-based 2D models and 3D brain organoid models that better represent and unravel the intricacies of neuropsychiatric disorder pathophysiology.

[Stem Cells and Development] To study the mechanisms in ESC self-renewal, screening and identification of key genes maintaining ESC self-renewal were performed by a genome-wide CRISPR-Cas9 knockout virus library.

[Cancer Discovery] Researchers interrogated gene expression and whole-genome CRISPR/Cas9 screening in a large panel of patient-derived glioblastoma (GBM) stem cells (GSCs), differentiated GBM cells, and neural stem cells to identify master regulators of GSC stemness.