CRISPR-Cas9

[Stem Cell Reports] Scientists reported on reprogramming of fibroblasts from two Duchenne muscular dystrophy mouse models into induced myogenic progenitor cells by MyoD overexpression in concert with small molecule treatment.

[Molecular Cancer] CRISPR-based loss-of-function genetic screens were used to target 18,053 protein-coding genes in hepatocellular carcinoma cells to identify chemotherapy-related synthetic lethal genes in these cells.

[Stem Cell Research] The authors generated a Beta2-microglobulin (B2M) homozygous knockout somatic cell nuclear transfer-induced ESC line using CRISPR/Cas9-mediated gene targeting.

[Oncogene] Investigators demonstrated that HMGN1 knockout-mitigated leukemic phenotypes including hepatosplenomegaly, thrombocytopenia, and anemia, were commonly observed in leukemia patients, and significantly increased survival in vivo.

[Science Immunology] Researchers showed that SOCS1 was a critical node integrating both IL-2 and IFN-γ signals to block multiple downstream signaling pathways abrogating CD4+ T helper 1 (TH1) cell response.

[Journal of the American Society of Nephrology] The authors designed a non-human leukocyte antigen (HLA) antibody detection immunoassay using HLA class I and II–deficient glomerular endothelial cells that had been previously generated through CRISPR/Cas9-induced B2M and CIITA gene disruption.