A Polycomb Domain Found in Committed Cells Impairs Differentiation When Introduced into PRC1 in Pluripotent Cells

ESCs expressing a chimeric CBX7 were impaired in their ability to properly form embryoid bodies and neural progenitor cells and showed reduced activation of lineage-specific genes across differentiation.
[Molecular Cell]
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Nuclear Transporter Importin-13 Plays a Key Role in the Oxidative Stress Transcriptional Response

Scientists performed transcriptomic analysis on wild-type and Importin-13-knockout mouse ESCs, revealing enrichment of differentially expressed genes involved in stress responses and apoptosis regulation.
[Nature Communications]
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Distinct Roles of Haspin in Stem Cell Division and Male Gametogenesis

Researchers showed that mouse ESCs that lacked or overexpressed haspin, albeit prone to chromosome misalignment during metaphase, could still divide, expand and differentiate.
[Scientific Reports]
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Loss of Resf1 Reduces the Efficiency of Embryonic Stem Cell Self-Renewal and Germline Entry

The authors used Resf1 knockout cell lines to determine the requirements of RESF1 for ESC self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells
[Life Science Alliance]
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Gadd45g Initiates Embryonic Stem Cell Differentiation and Inhibits Breast Cell Carcinogenesis

Researchers showed that the transcripts of Gadd45 family genes, including Gadd45a, Gadd45b, and Gadd45g, were gradually increased upon mouse ESC differentiation. Upregulation of Gadd45 members decreased cell proliferation and induced endodermal and trophectodermal lineages.
[Cell Death Discovery]
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Improved Differentiation of hESC-Derived Pancreatic Progenitors by Using Human Fetal Pancreatic Mesenchymal Cells in a Micro‐Scalable Three-Dimensional Co-Culture System

The impact of 3D co-culture of human fetal pancreatic-derived mesenchymal cells with human embryonic stem cell-derived pancreatic progenitors development towards endocrine and beta cells was assessed.
[Stem Cell Reviews and Reports]
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Interactome Analysis Reveals Endocytosis and Membrane Recycling of EpCAM during Differentiation of Embryonic Stem Cells and Carcinoma Cells

Potential interactors of epithelial cell adhesion molecule (EpCAM) were identified in murine F9 teratoma cells using a SILAC-based proteomic approach. Endocytosis and membrane recycling were means of regulation of EpCAM protein levels during differentiation of ESCs and epithelial-to-mesenchymal transition induction in carcinoma cells.
[iScience]
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A Scaffold-Free Approach to Cartilage Tissue Generation Using Human Embryonic Stem Cells

Researchers developed a robust method to generate 3D, scaffold-free, hyaline cartilage tissue constructs from human ESCs that were composed of numerous chondrocytes in lacunae, embedded in an extracellular matrix containing Type II collagen, sulphated glycosaminoglycans and Aggrecan.
[Scientific Reports]
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Single-Cell Landscape of Nuclear Configuration and Gene Expression during Stem Cell Differentiation and X Inactivation

The authors followed changes in gene expression, chromatin accessibility, and nuclear structure associated with mouse ESC differentiation and X inactivation by integrating allele-specific data from these three modalities obtained by high-throughput single-cell RNA-seq, ATAC-seq, and Hi-C.
[Genome Biology]
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Construction of Extracellular Matrix-Based 3D Hydrogel and Its Effects on Cardiomyocytes

Researchers studied the construction of cardiac ECM-based 3D hydrogel and its effects on cell behaviors. The results indicated that cellular compartments could be efficiently removed from heart tissue via sodium dodecyl sulfonate- and Triton X-100-mediated decellularization, remaining the natural fibrous network structure and major proteins.
[Experimental Cell Research]
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Cell Differentiation in the Cardiac Embryonic Stem Cell Test (ESTc) Is Influenced by the Oxygen Tension in Its Underlying Embryonic Stem Cell Culture

Scientists investigated the chemical sensitivity of the ESTc carried out under 20% O2, using ESCs cultured under either 20% O2 or 5% O2. ESC viability was more sensitive to valproic acid but less sensitive to flusilazole when cultured under 5% versus 20% O2.
[Toxicology in Vitro]
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