embryonic stem cells

[Nucleic Acids Research] Researchers reported that deadenylase Cnot8 of Ccr4-Not complex played essential roles during the transition from naïve to formative state. Knock out (KO) Cnot8 resulted in early embryonic lethality in mice, but Cnot8 KO ESCs could be established.

[Open Biology] Investigators discuss the role of epigenetic and metabolic dysregulations and their cross-talk in tumorigenesis with a special focus on gliomagenesis.

[Experimental and Molecular Medicine] To enhance the maturation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs), the authors identified a natural steroidal alkaloid, tomatidine, as a new substance that stimulated the maturation of hESC-CMs.

[Differentiation] The pluripotency of mESCs was significantly reduced through extracellular signal-regulated kinase 1/2 phosphorylation upon knockdown of Maged1, and through G1 cell cycle arrest during cell division, resulting in significantly reduced mESC proliferation.

[Stem Cell Reports] By performing a CRISPR-based loss-of-function screen in ESCs, researchers uncovered a previously unappreciated functional redundancy among Polycomb repressive complex 1 subcomplexes.

[Cell Death & Disease] Scientists used a forward genetic approach with mouse haploid ESCs to generate high-throughput mutant libraries, from which numerous oxidative stress-targeting genes were screened out.