flow cytometry

[Bioengineered] Exosomes were extracted from the supernatant of the bone-marrow MSCs. The expression of the micro-RNA miR-93-3p was determined by qRT-PCR analysis.

[iScience] The authors showed that cultured HSCs expressed mast cell-related genes including Cd244 and suggested CD244 was a potent marker to exclude non-functional HSCs after in vitro culture thereby useful to elucidate mechanism of functional decline of HSCs during ex vivo treatment.

[Environmental Toxicology] HK-2 cell viability and apoptosis were detected by Cell Counting Kit-8 assay and flow cytometry analysis, respectively. β-amyrin was found to ameliorate kidney injury in diabetic nephropathy mice and suppressed inflammatory response as well as apoptosis of HG-stimulated HK-2 cells.

[Molecular Medicine Reports] Scientists investigated the involvement of Interferon regulatory factor (IRF) 2 in myocardial infarction (MI) and the underlying mechanism of IRF2 in pyroptosis. To mimic MI, ligation of the left anterior descending coronary artery was performed to establish an in vivo mouse model and rat cardiomyocytes H9c2 cells were cultured under hypoxic conditions to establish an in vitro model.

[Pediatric Research] Scientists evaluated the effect of hydroxyurea on immune profiles through a controlled prospective cohort study conducted in 30 children with sickle cell anemia and 30 healthy age-matched controls.

[Signal Transduction and Targeted Therapy] The authors combined analyses of immune cells and cytokine/chemokine networks with endothelial activation and injury.