hematopoietic stem cells

[Stem Cell Research & Therapy] Researchers generated iPSCs from 3 different GS-2 patients using lentiviral vectors. The iPSCs were characterized using flow cytometry and RT-PCR and tested for the expression of pluripotency markers. In vivo differentiation to cells from all three germlines was tested using a teratoma assay. In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media.

[Leukemia] Scientists identified the EVL/MIR342 gene locus as a hotspot for therapeutic vector insertions indicating its accessibility and expression in human hematopoietic stem and progenitor cells.

[Leukemia] Luspatercept pre-treatment of mesenchymal stromal cells increased the subsequent homing of co-cultured hematopoietic stem and progenitor cells in zebrafish embryos.

[Cytotherapy] cGMP valproic acid-mediated expansion was initiated with CD34+ cells isolated from cryopreserved primary umbilical cord blood collections, and the functionality after a second cryopreservation step of the expanded product evaluted in vitro and in mouse xenografts.

[Leukemia] After transfer of human cord blood-derived hematopoietic stem and progenitor cells, transgenic mice on the NSGW41 background, termed NSGW41hIL7, showed elevated and prolonged human cellularity in the thymus.

[Leukemia] The authors investigated whether luspatercept could modulate the functional properties of MSCs as key components of the bone marrow microenvironment.