intestinal epithelial cells

[Nature Communications] Scientists developed a live cell ratiometric fluorescent Optical Stem Cell Activity Reporter (OSCAR). Using the small intestinal crypt as a model, OSCAR revealed in real time the dynamics of dormancy induction and cellular differentiation in vitro, and allowed for the identification and isolation of several populations of transcriptionally diverse OSCARhigh and OSCARlow intestinal epithelial cell states in vivo.

[Scientific Reports] Investigators generated CYP3A4-expressing Caco-2 cells and attempted to establish a model that could simultaneously evaluate drug absorption and metabolism.

[Science Advances] Researchers developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable human intestinal epithelial cells (hIECs) progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4.

[Stem Cell Research & Therapy] Cecal ligation and puncture-induced sepsis rats and lipopolysaccharide-stimulated intestinal epithelial cells to investigate the effect of mesenchymal stem cell-derived microvesicles on intestinal barrier dysfunction.

[Cell Host & Microbe] In the intestine, Salmonella enterica serovar Typhimurium actively invaded intestinal epithelial cells and survived in the Salmonella-containing vacuole and the cell cytosol.

[Proceedings of the National Academy of Sciences of the United States of America] Using intestinal organoids, researchers showed that concomitant activation of Notch signaling and ablation of p53 induce a highly proliferative and regenerative cell state, which was associated with increased levels of Yap and the histone methyltransferase Mll1.