leukocytes

[Nature Protocols] Researchers presented a multiplexed mass cytometry protocol for single-cell analysis of post-translational modification signaling and cell states in organoids and organoids co-cultured with fibroblasts and leukocytes.

[Stem Cell Research & Therapy] Modified mixed leukocyte reaction was performed between feline adipose-derived MSCs and PBMCs. Changes of cell cycle stages, phenotype and cellular senescence were determined through flow cytometry and gene expression analysis.

[Cellular & Molecular Immunology] Scientists discuss the role of inflammation in initiating epigenetic alterations in immune cells, cancer-associated fibroblasts, and cancer cells and suggest how and when epigenetic interventions can be combined with immunotherapies to improve therapeutic outcomes.

[Rocket Pharmaceuticals, Inc.] Rocket Pharmaceuticals, Inc. announced that the US FDA has lifted the clinical hold on the company’s Phase I clinical trial of RP-A501 for the treatment of Danon Disease, allowing patient enrollment to resume. RP-A501 is an investigational gene therapy product being developed for Danon Disease and the first potential gene therapy for monogenic heart failure.

[Hypertension] Scientists confirmed that EPAS1-specific inhibitor PT2385 ameliorated HPH features, as demonstrated by right ventricle hypertrophy, right ventricular systolic pressure, and pulmonary vascular remodeling.

[Cell Reports] Scientists reported that pro-inflammatory β cell small extracellular vesicles induced β cell dysfunction, promoted a pro-inflammatory islet transcriptome, and enhanced recruitment of CD8+ T cells and macrophages.