mass spectrometry

[Circulation Research] Using LysMCre-mediated mammalian STE20-like protein kinase 1/2 (Mst1/2)-deficient mice, researchers found that MST1 deficiency exacerbated cardiac dysfunction after myocardial infarction.

[Pharmaceutical Biology] The effect of C. sativus fruit extract on intestinal chloride secretion was investigated in human colonic T84 cells, ex vivo mouse intestinal tissue using an Ussing chamber, and the two-electrode voltage-clamp technique to record calcium sensitive TMEM16A chloride currents in Xenopus laevis oocytes.

[eLife] To gain functional insights into these tissue-resident intestinal intraepithelial T lymphocytes, the authors used high-resolution, quantitative mass spectrometry to compare the proteomes of induced tissue-resident intestinal intraepithelial T lymphocytes (T-IEL) and natural T-IEL subsets, with naive CD8+ T cells from lymph nodes.

[PLOS One] Investigators sampled four different collagen samples using standard mass spectrometry (MS) and MS/MS techniques.

[Journal of Biological Chemistry] Investigators summarized the different ionization methods, sample preparation steps and data analysis methods of mass spectrometry imaging (MSI) and focuses on several of the latest applications of matrix-assisted laser desorption/ionization MSI for drug studies in spheroids and organoids.

[Scientific Reports] Researchers performed untargeted ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry-based polar metabolomics and lipidomics to non-transformed and transformed colon cell lines as well as tissue samples from ten colorectal cancer patients.