Human hematopoietic stem cells were differentiated into megakaryocytes, enabling the transfection of miR-204-5p and the recovery of subsequent platelet-like structures.
[Thrombosis and Haemostasis]
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Scientists fabricated coaxial extruded core–shell tubular scaffolds and extrusion-based bioprinted cell-laden mesh scaffolds to mimic the functional niche in vitro.
[ACS Biomaterials Science & Engineering]
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The authors characterized the human hematopoietic stem cell and progenitor microenvironment. They obtained three adjacent bone marrow sections from ten healthy volunteers.
Investigators present a high throughput strategy for identifying and isolating megakaryocytes and their progenitor cells from a heterogeneous population of bone marrow samples.
Scientists highlight the coordinated immune response that contributes to COVID-19 pathogenesis and reveals discrete cellular components that can be targeted for therapy.
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Stephenson, E., Reynolds, G., Botting, R. A., Calero-Nieto, F. J., Morgan, M. D., Tuong, Z. K., Bach, K., Sungnak, W., Worlock, K. B., Yoshida, M., Kumasaka, N., Kania, K., Engelbert, J., Olabi, B., Spegarova, J. S., Wilson, N. K., Mende, N., Jardine, L., Gardner, L. C. S., … Haniffa, M. (2021). Single-cell multi-omics analysis of the immune response in COVID-19. Nature Medicine, 1–13. https://doi.org/10.1038/s41591-021-01329-2 Cite
iPSCs were reprogrammed from peripheral blood mononuclear cells and megakaryocytes were derived from the iPSCs in 194 healthy European American and African American subjects. mRNA was isolated and gene expression measured by RNA sequencing. Protein expression was measured in 62 of the subjects using mass spectrometry.
[Journal of Thrombosis and Haemostasis]
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Sequential waves of definitive hematopoiesis arise from yolk sac and intembryonic hemogenic endothelia through an endothelial-to-hematopoietic transition.
Scientists defined an optimal, stepwise and completely xeno‐free culture protocol for the generation of megakaryocytes from human embryonic stem cells.
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Zhang, B., Wu, X., Zi, G., He, L., Wang, S., Chen, L., Fan, Z., Nan, X., Xi, J., Yue, W., Wang, L., Wang, L., Hao, J., Pei, X., & Li, Y. (n.d.). Large-scale generation of megakaryocytes from human embryonic stem cells using transgene-free and stepwise defined suspension culture conditions. Cell Proliferation, n/a(n/a), e13002. https://doi.org/https://doi.org/10.1111/cpr.13002 Cite
Single-cell gene expression profiling with parallel mutation detection demonstrated that the megakaryocyte-primed hematopoietic stem cells subpopulation expanded significantly with enhanced potential in untreated individuals with JAK2V617F+essential thrombocythemia, driven primarily by the JAK2 mutation and elevated interferon signaling.
[Cell Stem Cell]
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Tong, J., Sun, T., Ma, S., Zhao, Y., Ju, M., Gao, Y., Zhu, P., Tan, P., Fu, R., Zhang, A., Wang, D., Wang, D., Xiao, Z., Zhou, J., Yang, R., Loughran, S. J., Li, J., Green, A. R., Bresnick, E. H., … Shi, L. (2021). Hematopoietic Stem Cell Heterogeneity Is Linked to the Initiation and Therapeutic Response of Myeloproliferative Neoplasms. Cell Stem Cell, 0(0). https://doi.org/10.1016/j.stem.2021.01.018 Cite
The authors demonstratef that the splenic microenvironment, specifically 15-PGDH high-expressing macrophages, megakaryocytes, and mast cells, regulated steady-state hematopoiesis and potentiated recovery after bone-marrow transplantation.
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Smith, J. N. P., Dawson, D. M., Christo, K. F., Jogasuria, A. P., Cameron, M. J., Antczak, M. I., Ready, J. M., Gerson, S. L., Markowitz, S. D., & Desai, A. B. (2021). 15-PGDH inhibition activates the splenic niche to promote hematopoietic regeneration. JCI Insight. https://doi.org/10.1172/jci.insight.143658 Cite
Human cord blood-derived megakaryocytes were treated with antisense oligonucleotides (ASOs) to study effects on proplatelet production. Platelet activation and platelet-leukocyte aggregates were analyzed in ASO-treated blood from healthy human volunteers.
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Slingsby, M. H. L., Vijey, P., Tsai, I.-T., Roweth, H., Couldwell, G., Wilkie, A. R., Gaus, H., Goolsby, J. M., Okazaki, R., Terkovich, B. E., Semple, J. W., Thon, J. N., Henry, S. P., Narayanan, P., & Jr, J. E. I. (2020). Sequence-specific 2’-O-methoxyethyl antisense oligonucleotides activate human platelets through glycoprotein VI, triggering formation of plateletleukocyte aggregates. Haematologica. https://doi.org/10.3324/haematol.2020.260059 Cite