Researchers adapted a centrifugation-based method for the isolation of murine bone marrow and compared it to the traditional flushing method. Analysis of primary hematopoietic stem cells, immune cells, and megakaryocytes revealed a comparable distribution of cellular (sub)populations.
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Heib, T., Gross, C., Müller, M.-L., Stegner, D., & Pleines, I. (2020). Isolation of murine bone marrow by centrifugation or flushing for the analysis of hematopoietic cells – a comparative study. Platelets. https://www.tandfonline.com/doi/abs/10.1080/09537104.2020.1797323 Cite