Isolation of Murine Bone Marrow by Centrifugation or Flushing for the Analysis of Hematopoietic Cells – A Comparative Study

Researchers adapted a centrifugation-based method for the isolation of murine bone marrow and compared it to the traditional flushing method. Analysis of primary hematopoietic stem cells, immune cells, and megakaryocytes revealed a comparable distribution of cellular (sub)populations.
[Platelets]
Heib, T., Gross, C., Müller, M.-L., Stegner, D., & Pleines, I. (2020). Isolation of murine bone marrow by centrifugation or flushing for the analysis of hematopoietic cells – a comparative study. Platelets. https://www.tandfonline.com/doi/abs/10.1080/09537104.2020.1797323 Cite
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Increased CXCL4 Expression in Hematopoietic Cells Links Inflammation and Progression of Bone Marrow Fibrosis in MPN

The authors demonstrated that the absence of hematopoietic CXCL4 ameliorates the myeloproliferative neoplasm phenotype, reduces stromal cell activation and bone marrow fibrosis and decreases the activation of pro-fibrotic pathways in megakaryocytes, inflammation in fibrosis-driving cells and JAK/STAT activation in both megakaryocytes and stromal cells in three murine primary myelofibrosis models.
[Blood]
Gleitz, H., Dugourd, A. J. F., Leimkühler, N. B., Snoeren, I. A. M., Fuchs, S. N. R., Menzel, S., Ziegler, S., Kroeger, N., Triviai, I., Büsche, G., Kreipe, H., Banjanin, B., Pritchard, J., Hoogenboezem, R., Bindels, E., Schumacher, N., Rose-John, S., Elf, S., Saez-Rodriguez, J., … Schneider, R. K. (n.d.). Increased CXCL4 expression in hematopoietic cells links inflammation and progression of bone marrow fibrosis in MPN. Blood. https://doi.org/10.1182/blood.2019004095 Cite
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