Investigators determined how the microglial transcriptome was altered following modest changes to Spi1 expression in primary mouse microglia.
6445212 94VCEIEH items 1 apa default asc 1
Investigators found that high CD14+ cell content posed a challenge for manufacturing CAR T cells especially in patients with non-Hodgkin’s lymphoma and multiple myeloma due to the non-specific phagocytosis of the magnetic beads used to activate CD3+ T cells.
[Molecular Therapy-Methods & Clinical Development]
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Wang, X., Borquez-Ojeda, O., Stefanski, J., Du, F., Qu, J., Chaudhari, J., Thummar, K., Zhu, M., Shen, L., Hall, M., Gautam, P., Wang, Y., Senechal, B., Sikder, D., Adusumilli, P. S., Brentjens, R. J., Curran, K., Geyer, M. B., Mailankhody, S., … Rivière, I. (2021). Depletion of High-Content CD14+ Cells from Apheresis Products is Critical for the Successful Transduction and Expansion of CAR T cells during Large-scale cGMP Manufacturing. Molecular Therapy - Methods & Clinical Development, 0(0). https://doi.org/10.1016/j.omtm.2021.06.014 Cite
Bacteria injected into the organoid lumen entered umbrella-like cells and proliferated to form intracellular bacterial communities -like bodies.
6445212 83TIRZPJ items 1 apa default asc 1
Sharma, K., Thacker, V. V., Dhar, N., Cabrer, M. C., Dubois, A., Signorino-Gelo, F., Mullenders, J., Knott, G. W., Clevers, H., & McKinney, J. D. (2021). Early invasion of the bladder wall by solitary bacteria protects UPEC from antibiotics and neutrophil swarms in an organoid model. Cell Reports, 36(3). https://doi.org/10.1016/j.celrep.2021.109351 Cite
Neutrophils are immune cells with unusual biological features that furnish potent antimicrobial properties. These cells phagocytose and subsequently kill prokaryotic and eukaryotic organisms very efficiently.
Single-cell RNA sequencing and protected bone marrow chimeras demonstrated that ingestion of lipids led to specific transcriptional activation of a population of resident macrophages expressing Lyve1, Tim4, and ABCA1.
6445212 GKSY5KTJ items 1 apa default asc 1
Magalhaes, M. S., Smith, P., Portman, J. R., Jackson-Jones, L. H., Bain, C. C., Ramachandran, P., Michalidou, Z., Stimson, R. H., Dweck, M. R., Denby, L., Henderson, N. C., Jenkins, S. J., & Bénézech, C. (2021). Role of Tim4 in the regulation of ABCA1+ adipose tissue macrophages and post-prandial cholesterol levels. Nature Communications, 12(1), 4434. https://doi.org/10.1038/s41467-021-24684-7 Cite
In mice, lack of PTPN22 augmented antitumor activity with greater infiltration and activation of macrophages, natural killer (NK) cells, and T cells.
[Journal of Clinical Investigation]
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Ho, W. J., Croessmann, S., Lin, J., Phyo, Z. H., Charmsaz, S., Danilova, L., Mohan, A. A., Gross, N. E., Chen, F., Dong, J., Aggarwal, D., Bai, Y., Wang, J., He, J., Leatherman, J. M., Yarchoan, M., Armstrong, T. D., Zaidi, N., Fertig, E. J., … Jaffee, E. M. (2021). Systemic inhibition of PTPN22 augments anticancer immunity. The Journal of Clinical Investigation. https://doi.org/10.1172/JCI146950 Cite
A new strategy to modify MSCs to express specific chemokine receptors using mRNA engineering was developed to evaluate the homing ability of MSCs and its therapeutic effects for inflammatory bowel disease.
[Molecular Therapy-Nucleic Acids]
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Researchers hypothesized that dynamin-related protein 1 defects were a causal factor directly involved in liver disease development and stimulated liver disease progression.
6445212 U26ZXJIW items 1 apa default asc 1
Researchers investigated how ICG001 could prevent cardiac hypertrophy and fibrosis induced by transverse aortic constriction and found it may have mediated the substrates in the glycolysis pathway and the distinct alteration of oxidative stress during cardiac hypertrophy and heart failure.
7992332 HQXGB52M items 1 apa default asc 1
Researchers investigated the mechanism by which S100A9 was involved in neutrophil activation, neutrophil extracellular trap-induced airway inflammation, and macrophage polarization in neutrophilic asthma.
[Experimental & Molecular Medicine]
The authors reported the development of a highly sensitive and reproducible Bio-ELISA to quantify picogram levels of TH in multiple model systems. They applied this assay to monocytes isolated from blood of persons with Parkinson’s disease and to age-matched, healthy controls.
[npj Parkinson’s Disease]
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Gopinath, A., Badov, M., Francis, M., Shaw, G., Collins, A., Miller, D. R., Hansen, C. A., Mackie, P., Tansey, M. G., Dagra, A., Madorsky, I., Ramirez-Zamora, A., Okun, M. S., Streit, W. J., & Khoshbouei, H. (2021). TNFα increases tyrosine hydroxylase expression in human monocytes. Npj Parkinson’s Disease, 7(1), 1–15. https://doi.org/10.1038/s41531-021-00201-x Cite