Tag Archives: qRT-PCR

CGRP Protects Bladder Smooth Muscle Cells Stimulated By High Glucose through Inhibiting p38 MAPK Pathway In Vitro

Researchers indicated that calcitonin gene-related peptide (CGRP) protected bladder smooth muscle cells from oxidative stress induced by high glucose in vitro, and inhibit the α-SM-actin expression decrease through inhibiting the intracellular p38 MAPK signaling pathway.
[Scientific Reports]
Xue, J., Liu, Y., Zhang, S., Ding, L., Shen, B., Shao, Y., & Wei, Z. (2021). CGRP protects bladder smooth muscle cells stimulated by high glucose through inhibiting p38 MAPK pathway in vitro. Scientific Reports, 11(1), 7643. https://doi.org/10.1038/s41598-021-87140-y Cite
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MiR-485 Targets the DTX4 Gene to Regulate Milk Fat Synthesis in Bovine Mammary Epithelial Cells

The relationship between the candidate target gene DTX4 and miR-485 was validated by bioinformatics and real-time fluorescent quantitative PCR and Western blot analyses in bovine mammary epithelial cells.
[Scientific Reports]
Liu, J., Jiang, P., Iqbal, A., Ali, S., Gao, Z., Pan, Z., Xia, L., Yin, F., & Zhao, Z. (2021). MiR-485 targets the DTX4 gene to regulate milk fat synthesis in bovine mammary epithelial cells. Scientific Reports, 11(1), 7623. https://doi.org/10.1038/s41598-021-87139-5 Cite
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Hypoxic Glioma-Derived Exosomes Promote M2-Like Macrophage Polarization by Enhancing Autophagy Induction

The authors investigated the effects of glioma-derived exosomes on autophagy, the polarization of tumor-associated macrophages, and glioma progression.
[Cell Death & Disease]
Xu, J., Zhang, J., Zhang, Z., Gao, Z., Qi, Y., Qiu, W., Pan, Z., Guo, Q., Li, B., Zhao, S., Guo, X., Qian, M., Chen, Z., Wang, S., Gao, X., Zhang, S., Wang, H., Guo, X., Zhang, P., … Li, G. (2021). Hypoxic glioma-derived exosomes promote M2-like macrophage polarization by enhancing autophagy induction. Cell Death & Disease, 12(4), 1–16. https://doi.org/10.1038/s41419-021-03664-1 Cite
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Exosomes from Human Umbilical Cord Mesenchymal Stem Cells Attenuate the Inflammation of Severe Steroid-Resistant Asthma by Reshaping Macrophage Polarization

Investigators evaluated the effects of MSC-exosomes on airway hyper responsiveness, inflammation, histopathology, and macrophage polarization in severe, steroid-resistant asthma in mice.
[Stem Cell Research & Therapy]
Dong, B., Wang, C., Zhang, J., Zhang, J., Gu, Y., Guo, X., Zuo, X., Pan, H., Hsu, A. C.-Y., Wang, G., & Wang, F. (2021). Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization. Stem Cell Research & Therapy, 12(1), 204. https://doi.org/10.1186/s13287-021-02244-6 Cite
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The Hepatokine Fetuin-A Disrupts Functional Maturation of Pancreatic Beta Cells

The effects of fetuin-A were assessed during in vitro maturation of porcine neonatal islet cell clusters and in adult human islets. Expression alterations were examined via microarray, RNA sequencing and reverse transcription quantitative real-time PCR, proteins were analyzed by western blotting and immunostaining, and insulin secretion was quantified in static incubations.
[Diabetologia]
The hepatokine fetuin-A disrupts functional maturation of pancreatic beta cells | SpringerLink. (n.d.). Retrieved March 30, 2021, from https://link.springer.com/article/10.1007%2Fs00125-021-05435-1 Cite
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Peptide-Based Scaffolds for the Culture and Maintenance of Primary Human Hepatocytes

Researchers report the use of a nanofibrous hydrogel as a 3D scaffold for the culture and maintenance of functional primary human hepatocytes.
[Scientific Reports]
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METTL3-Mediated m6a Methylation of SPHK2 Promotes Gastric Cancer Progression by Targeting KLF2

Quantitative real-time PCR, western blot and immunohistochemistry were utilized to detect the expression profiles and prognostic significance of SPHK2 in gastric cancer.
[Oncogene]
Huo, F.-C., Zhu, Z.-M., Zhu, W.-T., Du, Q.-Y., Liang, J., & Mou, J. (2021). METTL3-mediated m 6 A methylation of SPHK2 promotes gastric cancer progression by targeting KLF2. Oncogene, 1–14. https://doi.org/10.1038/s41388-021-01753-1 Cite
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Oleandrin, a Cardiac Glycoside, Induces Immunogenic Cell Death via the PERK/elF2α/ATF4/CHOP Pathway in Breast Cancer

Researchers showed that oleandrin treatment triggered breast cancer cell immunogenic cell death by inducing calreticulin exposure on cell surface and the release of high-mobility group protein B1, heat shock protein 70/90, and adenosine triphosphate.
[Cell Death & Disease]
Li, X., Zheng, J., Chen, S., Meng, F., Ning, J., & Sun, S. (2021). Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2α/ATF4/CHOP pathway in breast cancer. Cell Death & Disease, 12(4), 1–15. https://doi.org/10.1038/s41419-021-03605-y Cite
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Photobiomodulation Therapy Increases Collagen II after Tendon Experimental Injury

Photobiomodulation effectively interfered in tendon tissue repair after injury by stimulating mesenchymal cell proliferation and the synthesis of collagen type II, which was suggested to provide structural support to the interstitial tissues during the healing process of the Achilles tendon.
[Histology and Histopathology]
Photobiomodulation therapy increases collagen II after tendon experimental injury. (2021). Histology and Histopathology, 1. https://doi.org/10.14670/HH-18-330 Cite
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Nifuroxazide Improves Insulin Secretion and Attenuates High Glucose-Induced Inflammation and Apoptosis in INS-1 Cells

The authors used high concentration glucose-induced INS-1 cells to examine the effects of Nifuroxazide on high glucose-induced cell function by glucose-stimulated insulin secretion.
[European Journal of Pharmacology]
Liu, J.-Y., Zhang, Y.-C., Xie, R.-R., Song, L.-N., Yang, W.-L., Xin, Z., Cao, X., & Yang, J.-K. (2021). Nifuroxazide improves insulin secretion and attenuates high glucose-induced inflammation and apoptosis in INS-1 cells. European Journal of Pharmacology, 174042. https://doi.org/10.1016/j.ejphar.2021.174042 Cite
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Islet Sympathetic Innervation and Islet Neuropathology in Patients with Type 1 Diabetes

To investigate islet sympathetic innervation in type 1 diabetes (T1D), sympathetic tyrosine hydroxylase axons were analyzed in control non-diabetic organ donors, non-diabetic islet autoantibody-positive individuals, and age-matched persons with T1D.
[Scientific Reports]
Campbell-Thompson, M., Butterworth, E. A., Boatwright, J. L., Nair, M. A., Nasif, L. H., Nasif, K., Revell, A. Y., Riva, A., Mathews, C. E., Gerling, I. C., Schatz, D. A., & Atkinson, M. A. (2021). Islet sympathetic innervation and islet neuropathology in patients with type 1 diabetes. Scientific Reports, 11(1), 6562. https://doi.org/10.1038/s41598-021-85659-8 Cite
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