Tag Archives: qRT-PCR

LncRNA GAS6-AS2 Promotes Non-Small-Cell Lung Cancer Cell Proliferation via Regulating miR-144-3p/ MAPK6 Axis

GAS6-AS2 was identified, and its roles as well as mechanisms in regulating proliferation of non-small cell lung cancer cells were investigated.
[Cell Cycle]
Hou, G., Yang, J., Tang, J., & He, Y. (2021). LncRNA GAS6-AS2 promotes non-small-cell lung cancer cell proliferation via regulating miR-144-3p/ MAPK6 axis. Cell Cycle, 0(0), 1–15. https://doi.org/10.1080/15384101.2020.1867782 Cite
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MicroRNA-494-3p Prevents Liver Fibrosis and Attenuates Hepatic Stellate Cell Activation by Inhibiting Proliferation and Inducing Apoptosis through Targeting TRAF3

Alcoholic hepatitis mice model and primarily cultured mice hepatic stellate cells model were constructed. Levels of aspartate aminotransferase and alanine aminotransferase were analyzed by ELISA.
[Annals of Hepatology]
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Identification of miR-320 Family Members as Potential Diagnostic and Prognostic Biomarkers in Myelodysplastic Syndromes

Scientists identified novel potential microRNAs bound up with the diagnosis and prognosis of myelodysplastic syndromes (MDS). miRNA microarray analysis was used to screen deregulated microRNAs in the bone marrow of MDS patients.
[Scientific Reports]
Wan, C., Wen, J., Liang, X., Xie, Q., Wu, W., Wu, M., & Liu, Z. (2021). Identification of miR-320 family members as potential diagnostic and prognostic biomarkers in myelodysplastic syndromes. Scientific Reports, 11(1), 183. https://doi.org/10.1038/s41598-020-80571-z Cite
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Exosomal LINC00161 Promotes Angiogenesis and Metastasis via Regulating miR-590-3p/ROCK Axis in Hepatocellular Carcinoma

Functional study demonstrated that exosomal LINC00161 derived from hepatocellular carcinoma (HCC)-cells were significantly associated with enhanced proliferation, migration, and angiogenesis in HUVECs in vitro, all of which were effectively inhibited when LINC00161 was sliced with shRNA in HCC-cells.
[Cancer Gene Therapy]
You, L.-N., Tai, Q.-W., Xu, L., Hao, Y., Guo, W.-J., Zhang, Q., Tong, Q., Zhang, H., & Huang, W.-K. (2021). Exosomal LINC00161 promotes angiogenesis and metastasis via regulating miR-590-3p/ROCK axis in hepatocellular carcinoma. Cancer Gene Therapy, 1–18. https://doi.org/10.1038/s41417-020-00269-2 Cite
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CircNOL10 Suppresses Breast Cancer Progression by Sponging miR-767-5p To Regulate SOCS2/JAK/STAT Signaling

Scientists investigated the biological functions of circNOL10 and its potential molecular mechanisms in breast cancer.
[Journal of Biomedical Science]
Wang, F., Wang, X., Li, J., Lv, P., Han, M., Li, L., Chen, Z., Dong, L., Wang, N., & Gu, Y. (2021). CircNOL10 suppresses breast cancer progression by sponging miR-767-5p to regulate SOCS2/JAK/STAT signaling. Journal of Biomedical Science, 28(1), 4. https://doi.org/10.1186/s12929-020-00697-0 Cite
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Breast Cancer Exosomes Contribute to Pre-Metastatic Niche Formation and Promote Bone Metastasis of Tumor Cells

Researchers indicated that breast cancer cell-derived exosomes played an important role in promoting breast cancer bone metastasis, which was associated with the formation of pre-metastatic niche via transferring miR-21 to osteoclasts.
[Theranostics]
Yuan, X., Qian, N., Ling, S., Li, Y., Sun, W., Li, J., Du, R., Zhong, G., Liu, C., Yu, G., Cao, D., Liu, Z., Wang, Y., Qi, Z., Yao, Y., Wang, F., Liu, J., Hao, S., Jin, X., … Li, Y. (2021). Breast cancer exosomes contribute to pre-metastatic niche formation and promote bone metastasis of tumor cells. Theranostics, 11(3), 1429–1445. https://doi.org/10.7150/thno.45351 Cite
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Cannabinoid Receptor Activation on Hematopoietic Cells and Enterocytes Protects against Colitis

Clinical parameters of colitis were assessed by colonoscopy, histology, flow cytometry and detection of serum biomarkers; single-cell RNA sequencing and qRT-PCR were used to evaluate the effects of cannabinoids on enterocytes.
[Journal of Crohns & Colitis]
Becker, W., Alrafas, H. R., Busbee, P. B., Walla, M. D., Wilson, K., Miranda, K., Cai, G., Putluri, V., Putluri, N., Nagarkatti, M., & Nagarkatti, P. S. (2020). Cannabinoid receptor activation on hematopoietic cells and enterocytes protects against colitis. Journal of Crohn’s and Colitis, jjaa253. https://doi.org/10.1093/ecco-jcc/jjaa253 Cite
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Circ6401, a Novel Circular RNA, Is Implicated in Repair of the Damaged Endometrium by Wharton’s Jelly-Derived Mesenchymal Stem Cells through Regulation of the miR-29b-1-5p/RAP1B Axis

Wharton’s jelly (WJ)-MSCs were cocultured with or without endometrial stromal cells (ESCs) damaged by mifepristone. TUNEL staining assays, EdU proliferation assays, flow cytometry apoptosis assays, and western blot assays were performed to observe the reparative effect of WJ-MSCs on damaged ESCs.
[Stem Cell Research & Therapy]
Shi, Q., Sun, B., Wang, D., Zhu, Y., Zhao, X., Yang, X., & Zhang, Y. (2020). Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton’s jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis. Stem Cell Research & Therapy, 11(1), 520. https://doi.org/10.1186/s13287-020-02027-5 Cite
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MicroRNA-155 Contributes to Plexiform Neurofibroma Growth Downstream of MEK

Scientists analyzed a microRNA (miR) microarray comparing with normal and plexiform neurofibroma Schwann cells (PNF-SCs) and identified differences in miR expression, and they validated in mouse PNFs versus normal mouse SCs by qRT-PCR.
[Oncogene]
Na, Y., Hall, A., Choi, K., Hu, L., Rose, J., Coover, R. A., Miller, A., Hennigan, R. F., Dombi, E., Kim, M.-O., Subramanian, S., Ratner, N., & Wu, J. (2020). MicroRNA-155 contributes to plexiform neurofibroma growth downstream of MEK. Oncogene, 1–13. https://doi.org/10.1038/s41388-020-01581-9 Cite
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Metabolic Reprogramming and Predominance of Solute Carrier Genes during Acquired Enzalutamide Resistance in Prostate Cancer

The transcriptomic profile of paired enzalutamide-sensitive and resistant LNCaP and C4-2B prostate cancer cells were compared for identification of genes involved in drug resistance by performing an unbiased bioinformatics analysis and further validation. Next-Gen sequencing detected 9409 and 7757 genes differentially expressed in LNCaP and C4-2B cells, compared to their parental counterparts.
[Cells]
Verma, S., Shankar, E., Chan, E. R., & Gupta, S. (2020). Metabolic Reprogramming and Predominance of Solute Carrier Genes during Acquired Enzalutamide Resistance in Prostate Cancer. Cells, 9(12), 2535. https://doi.org/10.3390/cells9122535 Cite
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Microenvironmental Changes Induced by Placenta-Derived Mesenchymal Stem Cells Restore Ovarian Function in Ovariectomized Rats via Activation of the PI3K-FOXO3 Pathway

Blood and ovary tissue were collected and analyzed after various placenta -derived (PD)-MSC transplantation treatments in an ovariectomized rat model. Changes in the expression of folliculogenesis- and ovary regeneration-related genes induced by PD-MSC treatments were analyzed by qRT-PCR, Western blotting, and histological analysis.
[Stem Cell Research & Therapy]
Choi, J. H., Seok, J., Lim, S. M., Kim, T. H., & Kim, G. J. (2020). Microenvironmental changes induced by placenta-derived mesenchymal stem cells restore ovarian function in ovariectomized rats via activation of the PI3K-FOXO3 pathway. Stem Cell Research & Therapy, 11(1), 486. https://doi.org/10.1186/s13287-020-02002-0 Cite
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