Tag Archives: qRT-PCR

Long Non-Coding RNA SNHG6 Regulates the Sensitivity of Prostate Cancer Cells to Paclitaxel by Sponging miR-186

Small nucleolar RNA host gene 6 (SNHG6) expression was increased and miR-186 expression was reduced in drug-resistant prostate cancer tissues and cells.
[Cancer Cell International]
Cao, C., Sun, G., & Liu, C. (2020). Long non-coding RNA SNHG6 regulates the sensitivity of prostate cancer cells to paclitaxel by sponging miR-186. Cancer Cell International, 20(1), 381. https://doi.org/10.1186/s12935-020-01462-x Cite
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LINC00992 Contributes to the Oncogenic Phenotypes in Prostate Cancer via Targeting miR-3935 and Augmenting GOLM1 Expression

Elevated levels of LINC00992 and GOLM1 were detected in prostate cancer tissues and cells. LINC00992 exerted facilitating functions in prostate cancer cell proliferation and migration.
[BMC Cancer]
Chen, J., Liu, X., Ke, K., Zou, J., Gao, Z., Habuchi, T., & Yang, X. (2020). LINC00992 contributes to the oncogenic phenotypes in prostate cancer via targeting miR-3935 and augmenting GOLM1 expression. BMC Cancer, 20(1), 749. https://doi.org/10.1186/s12885-020-07141-4 Cite
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FGL1 Regulates Acquired Resistance to Gefitinib by Inhibiting Apoptosis in Non-Small Cell Lung Cancer

Scientists investigated the role of fibrinogen-like protein 1 (FGL1) in regulating gefitinib resistance of PC9/GR non-small cell lung cancer.
[Respiratory Research]
Sun, C., Gao, W., Liu, J., Cheng, H., & Hao, J. (2020). FGL1 regulates acquired resistance to Gefitinib by inhibiting apoptosis in non-small cell lung cancer. Respiratory Research, 21(1), 210. https://doi.org/10.1186/s12931-020-01477-y Cite
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Overexpression of GPX3, a Potential Biomarker for Diagnosis and Prognosis of Breast Cancer, Inhibits Progression of Breast Cancer Cells In Vitro

Several databases were employed to perform in silico analyses for glutathione peroxidases (GPXs) family genes. qRT-PCR, western blot and immunohistochemistry staining were introduced to validate GPX3 expression in breast cancer.
[Cancer Cell International]
Lou, W., Ding, B., Wang, S., & Fu, P. (2020). Overexpression of GPX3, a potential biomarker for diagnosis and prognosis of breast cancer, inhibits progression of breast cancer cells in vitro. Cancer Cell International, 20(1), 378. https://doi.org/10.1186/s12935-020-01466-7 Cite
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circRACGAP1 Promotes Non-Small Cell Lung Cancer Proliferation by Regulating miR-144-5p/CDKL1 Signaling Pathway

Expression patterns of circRACGAP1 and miR-144-5p in non-small cell lung cancer tissues and cell lines were quantified by qRT-PCR analysis. Then, the function of circRACGAP1 on cell proliferation and tumorigenesis were confirmed in vitro and in vivo using CCK-8 assay, colony formation, EdU incorporation, and xenograft technique.
[Cancer Gene Therapy]
Lu, M., Xiong, H., Xia, Z.-K., Liu, B., Wu, F., Zhang, H.-X., Hu, C.-H., & Liu, P. (2020). circRACGAP1 promotes non-small cell lung cancer proliferation by regulating miR-144-5p/CDKL1 signaling pathway. Cancer Gene Therapy, 1–15. https://doi.org/10.1038/s41417-020-00209-0 Cite
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LncRNA KCNQ1OT1 Regulates the Invasion and Migration of Hepatocellular Carcinoma by Acting on S1PR1 through miR-149

A xenograft tumour assay was used to validate the role of KCNQ1OT1 in vivo. KCNQ1OT1 and S1PR1 were significantly increased, but miR-149 was decreased in HCC cells.
[Cancer Gene Therapy]
Cheng, J.-L., Li, D.-J., Lv, M.-Y., Pei, Y.-J., Zhang, X.-J., Li, L., Liu, X.-Y., & Fan, A.-H. (2020). LncRNA KCNQ1OT1 regulates the invasion and migration of hepatocellular carcinoma by acting on S1PR1 through miR-149. Cancer Gene Therapy, 1–14. https://doi.org/10.1038/s41417-020-0203-x Cite
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MicroRNA-148a-3p Inhibits Progression of Hepatocelluar Carcimoma by Repressing SMAD2 Expression in an Ago2 Dependent Manner

Investigators found that SMAD2 was highly expressed in hepatocellular carcinoma (HCC) and elevated SMAD2 expression predicted shorter overall survival time for HCC patients. SMAD2 promoted mobility and proliferation of HCC cells in vitro.
[Journal of Experimental & Clinical Cancer Research]
Huang, Z., Wen, J., Yu, J., Liao, J., Liu, S., Cai, N., Liang, H., Chen, X., Ding, Z., & Zhang, B. (2020). MicroRNA-148a-3p inhibits progression of hepatocelluar carcimoma by repressing SMAD2 expression in an Ago2 dependent manner. Journal of Experimental & Clinical Cancer Research, 39(1), 150. https://doi.org/10.1186/s13046-020-01649-0 Cite
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Long Non-Coding RNA MIR503HG Inhibits the Proliferation, Migration and Invasion of Colon Cancer Cells via MiR-107/Par4 Axis

MIR503HG negatively regulated its target miR-107. MiR-107 overexpression reversed the anti-tumor effects of MIR503HG overexpression on colon cancer cells. Par4 was a target of miR-107, which was positively regulated by MIR503HG. The promoting effects of MIR503HG silencing on colon cancer cells were eliminated by Par4 overexpression.
[Experimental Cell Research]
Han, H., Li, H., & Zhou, J. (2020). Long non-coding RNA MIR503HG inhibits the proliferation, migration and invasion of colon cancer cells via miR-107/Par4 axis. Experimental Cell Research, 395(2), 112205. https://doi.org/10.1016/j.yexcr.2020.112205 Cite
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LINC00324 Affects Non-Small Cell Lung Cancer Cell Proliferation and Invasion through Regulation of the miR-139-5p/IGF1R Axis

Scientists demonstrated whether LINC00324 participates in non-small cell lung cancer pathogenesis through other molecular mechanism.
[Molecular and Cellular Biochemistry]
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miR-3622a-3p Acts as a Tumor Suppressor in Colorectal Cancer by Reducing Stemness Features and EMT through Targeting Spalt-Like Transcription Factor 4

The effect of miR-3622a-3p on proliferation, apoptosis, cell cycle, migration and invasion of colorectal cancer (CRC) cells were investigated by a series of biological function assays and the results revealed that miR-3622a-3p could inhibit the malignant biological properties of CRC.
[Cell Death & Disease]
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EGF-Mediated Reduced miR-92a-1-5p Controls HTR-8/SVneo Cell Invasion through Activation of MAPK8 and FAS which in turn Increase MMP-2/-9 Expression

The binding of miR-92a-1-5p to MAPK8 and FAS 3′-UTR was confirmed by Luciferase reporter assay and Rescue assay. EGF increased MMP-2 & MMP-9 expression and reduced TIMP1 expression in HTR-8/SVneo cells.
[Scientific Reports]
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Enrichment Differentiation of Human Induced Pluripotent Stem Cells into Sinoatrial Node-Like Cells by Combined Modulation of BMP, FGF, and RA Signaling Pathways

Researchers investigated whether combined modulation of BMP, FGF, and RA signaling pathways could enrich the differentiation of sinoatrial node-like cells from human pluripotent stem cells.
[Stem Cell Research & Therapy]
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