Platelet lysate (PL) had been shown to induce chondrogenic differentiation of human umbilical cord‐derived MSCs Scientists investigated whether long non‐coding RNA H19 was involved in this process. Human umbilical cord MSCs were isolated, identified, and cultured in 5% PL‐supplemented chondrogenic differentiation medium
[Febs Open Bio]
miR-34a interacted with SIRT1 and suppressed SIRT1 expression. MiR-34a overexpression inhibited proliferation and promoted apoptosis of rat retinal vascular endothelial cells, which was effectively abolished by SIRT1 up-regulation.
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In vitro experiments (including colony formation, CCK-8, wound healing and transwell assays) and in vivo experiments indicated that down-regulation of SNHG10 significantly suppressed the proliferation and invasion of osteosarcoma cells
[Molecular Therapy-Nucleic Acids]
Investigators observed that plasmacytoma variant translocation 1 (PVT1) and caspase‐10 were upregulated while miR‐23a‐3p was downregulated in high glucose‐induced cardiomyocytes. High glucose repressed cardiomyocyte activity and induced cardiomyocyte apoptosis, but this influence was antagonized by PVT1 knockdown or miR‐23a‐3p overexpression.
[Cell Biology International]
Investigators showed that G3BP1 phosphorylation by casein kinase 2α (CK2α) triggered G3BP1 granule disassembly in injured axons. CK2α activity was temporally and spatially regulated by local translation of Csnk2a1 mRNA in axons after injury, but this required local translation of mTor mRNA and buffering of the elevated axonal Ca2+ that occurred after axotomy.
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Sahoo, P. K., Kar, A. N., Samra, N., Terenzio, M., Patel, P., Lee, S. J., Miller, S., Thames, E., Jones, B., Kawaguchi, R., Coppola, G., Fainzilber, M., & Twiss, J. L. (2020). A Ca2+-Dependent Switch Activates Axonal Casein Kinase 2α Translation and Drives G3BP1 Granule Disassembly for Axon Regeneration. Current Biology, 0(0). https://doi.org/10.1016/j.cub.2020.09.043 Cite
miR-124 or EZH2 was overexpressed in AsPC-1 and PANC1 cells. Then, the effects on cell viability, apoptosis, invasion, migration and epithelial mesenchymal transition were evaluated. The roles of miR-124 on the expression and function of EZH2 in pancreatic tumors were determined by dual luciferase reporter assay. Subsequently, miR-124 was transfected to bone marrow mesenchymal stromal cells (BM-MSCs), and the BM-MSCs derived exosomes were isolated and co-cultured with AsPC-1 and PANC1 cells, or injected into pancreatic cancer tumor-bearing mice.
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Bioinformatics binding site analysis, Luciferase reporter assay, Ago2 immunoprecipitation assays, RNA pull-down assay, RT-qPCR and Western blotting were utilized to demonstrate that mechanism of RP11-757G1.5 acts as a molecular sponge of miR-139-5p to regulate the expression of YAP1.
[Journal of Experimental & Clinical Cancer Research]
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Health, S. (n.d.). FDA authorizes new IND to evaluate impact of multi-strain probiotic DS-01TM on gut microbiota of patients with IBS. Retrieved October 8, 2020, from https://www.prnewswire.com/news-releases/fda-authorizes-new-ind-to-evaluate-impact-of-multi-strain-probiotic-ds-01-on-gut-microbiota-of-patients-with-ibs-301139955.html Cite
Investigators established a functional correlation between thymidylate synthase (TS), epithelial-to-mesenchymal transition (EMT), chemotherapy and metastasis and propose a network for TS mediated EMT.
[British Journal of Cancer]
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Siddiqui, M. A., Gollavilli, P. N., Ramesh, V., Parma, B., Schwab, A., Vazakidou, M. E., Natesan, R., Saatci, O., Rapa, I., Bironzo, P., Schuhwerk, H., Asangani, I. A., Sahin, O., Volante, M., & Ceppi, P. (2020). Thymidylate synthase drives the phenotypes of epithelial-to-mesenchymal transition in non-small cell lung cancer. British Journal of Cancer, 1–9. https://doi.org/10.1038/s41416-020-01095-x Cite
LINC00665 expression levels were determined using quantitative polymerase chain reaction analysis with breast cancer tissues and cell lines.
[Cellular & Molecular Biology Letters]
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Lv, M., Mao, Q., Li, J., Qiao, J., Chen, X., & Luo, S. (2020). Knockdown of LINC00665 inhibits proliferation and invasion of breast cancer via competitive binding of miR-3619-5p and inhibition of catenin beta 1. Cellular & Molecular Biology Letters, 25(1), 43. https://doi.org/10.1186/s11658-020-00235-8 Cite
The authors identified a long noncoding RNA LINC00857 that might regulate radio-sensitivity of lung adenocarcinoma cells.
[Molecular Therapy-Nucleic Acids]
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To elucidate the molecular mechanism of homeostasis in hypothermia and identify novel molecular markers to inform the diagnosis of fatal hypothermia, scientists focused on microRNA expression in skeletal muscle, which plays a role in cold-induced thermogenesis in mammals.
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Umehara, T., Kagawa, S., Tomida, A., Murase, T., Abe, Y., Shingu, K., & Ikematsu, K. (2020). Body temperature-dependent microRNA expression analysis in rats: rno-miR-374-5p regulates apoptosis in skeletal muscle cells via Mex3B under hypothermia. Scientific Reports, 10(1), 15432. https://doi.org/10.1038/s41598-020-71931-w Cite