reporter assay

[Journal of the American Heart Association] Researchers investigated the functional mechanism of exosomal microRNA‐96 derived from bone marrow mesenchymal stem cells in myocardial toxicity induced by doxorubicin.

[Therapeutic Advances in Chronic Disease] Real-time qualitative PCR and Western blot analysis were conducted to determine expression of HGF, PTEN, and osteoblast differentiation-related genes in ovariectomy-induced osteoporosis mice and the isolated bone marrow MSCs.

[Anti-Cancer Drugs] Psoriatic HaCat cells were used to investigate the function of NEAT1 and Galectin-7 as well as the effect and mechanism of paeoniflorin in psoriasis.

[Acta Biochimica et Biophysica Sinica] Researchers investigated the relationship between miR-144-3p and CEP55 expression. They evaluated the association between miR-144-3p and CEP55 expression and proliferation, invasion and apoptosis of non-small cell lung cancer cells.

[Journal of Bioenergetics and Biomembranes] The protein levels of osteogenesis-related genes, let-7i-5p, frizzled class receptor 4, Ki67, Wnt6 and β-catenin were measured by western blot assay. The levels of circ_FBLN1, FBLN1 mRNA and FZD4 mRNA were determined by quantitative real-time PCR assay.

[Molecular Medicine] The human umbilical cord MSC exosomes were found to effectively improve the viability of nucleus pulposus cells and protect them from pyroptosis through targeting METTL14, with a methyltransferase catalyzing m6A modification.