Circ_FBLN1 Promotes the Proliferation and Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Regulating Let-7i-5p/FZD4 Axis and Wnt/β-Catenin Pathway

The protein levels of osteogenesis-related genes, let-7i-5p, frizzled class receptor 4, Ki67, Wnt6 and β-catenin were measured by western blot assay. The levels of circ_FBLN1, FBLN1 mRNA and FZD4 mRNA were determined by quantitative real-time PCR assay.