Scientists studied TTA-A2 and paclitaxel in lung adenocarcinoma cell line- A549, cancer cells and human embryonic kidney cell line- HEK 293, control cell, in their monolayer and spheroids forms for viability, proliferation, morphology change, migration, and invasion-after 48–96 hours of treatment.
[Life Sciences]
1254621 {1254621:Y2HECUII} apa 50 1 153094 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-3e9185df69f5a1d58b337d53685893e7%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22Y2HECUII%22%2C%22library%22%3A%7B%22id%22%3A1254621%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kumari%20et%20al.%22%2C%22parsedDate%22%3A%222020-08-15%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKumari%2C%20N.%2C%20Bharagava%2C%20A.%2C%20%26amp%3B%20Rath%2C%20S.%20N.%20%282020%29.%20T-type%20calcium%20channel%20antagonist%2C%20TTA-A2%20exhibits%20anti-cancer%20properties%20in%203D%20spheroids%20of%20A549%2C%20a%20lung%20adenocarcinoma%20cell%20line.%20%3Ci%3ELife%20Sciences%3C%5C%2Fi%3E%2C%20118291.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.lfs.2020.118291%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.lfs.2020.118291%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D1254621%26amp%3Bitem_key%3DY2HECUII%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22T-type%20calcium%20channel%20antagonist%2C%20TTA-A2%20exhibits%20anti-cancer%20properties%20in%203D%20spheroids%20of%20A549%2C%20a%20lung%20adenocarcinoma%20cell%20line%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neema%22%2C%22lastName%22%3A%22Kumari%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anamika%22%2C%22lastName%22%3A%22Bharagava%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Subha%20Narayan%22%2C%22lastName%22%3A%22Rath%22%7D%5D%2C%22abstractNote%22%3A%22Aims%5CnDespite%20the%20advanced%20cancer%20treatments%2C%20there%20is%20increased%20resistance%20to%20chemotherapy%20and%20subsequent%20mortality.%20In%20lack%20of%20reliable%20data%20in%20monolayer%20cultures%20and%20animal%20models%2C%20researchers%20are%20shifting%20to%203D%20cancer%20spheroids%2C%20which%20represents%20the%20in%20vivo%20robust%20tumour%20morphology.%20Calcium%20is%20essential%20in%20cell%20signalling%20and%20proliferation.%20It%20is%20found%20that%20T-type%20calcium%20channels%20%28TTCCs%29%20are%20overexpressed%20in%20various%20cancer%20cells%2C%20supporting%20their%20increased%20proliferation.%20Many%20of%20the%20TTCCs%20blockers%20available%20could%20target%20other%20channels%20besides%20TTCCs%2C%20which%20can%20cause%20adverse%20effects.%20Therefore%2C%20we%20hypothesise%20that%20TTA-A2%2C%20a%20highly%20selective%20blocker%20towards%20TTCCs%2C%20can%20inhibit%20the%20growth%20of%20cancer%20spheroids%2C%20and%20provide%20an%20anti-cancer%20and%20an%20adjuvant%20role%20in%20cancer%20therapy.%5CnMethods%5CnWe%20studied%20TTA-A2%20and%20paclitaxel%20%28PTX-control%20drug%29%20in%20lung%20adenocarcinoma%20cell%20line-%20A549%2C%20cancer%20cells%20and%20human%20embryonic%20kidney%20cell%20line-%20HEK%20293%2C%20control%20cell%2C%20in%20their%20monolayer%20and%20spheroids%20forms%20for%20viability%2C%20proliferation%2C%20morphology%20change%2C%20migration%2C%20and%20invasion-after%2048%5Cu201396%5Cu202fh%20of%20treatment.%5CnKey%20findings%5CnThough%20the%20results%20varied%20between%20the%20monolayer%20and%20spheroids%20studies%2C%20we%20found%20both%20anti-cancer%20as%20well%20as%20adjuvant%20effect%20of%20TTA-A2%20in%20both%20the%20studies.%20TTA-A2%20was%20able%20to%20inhibit%20the%20growth%2C%20viability%2C%20and%20metastasis%20of%20the%20cancer%20cells%20and%20spheroids.%20Differences%20in%20the%20results%20of%20two%20modes%20might%20explain%20that%20why%20drugs%20tested%20successfully%20in%20monolayer%20culture%20fail%20in%20clinical%20trials.%5CnSignificance%5CnThis%20study%20establishes%20the%20role%20of%20TTA-A2%2C%20a%20potent%20TTCC%20blocker%20as%20an%20anti-cancer%20and%20adjuvant%20drug%20in%20reducing%20the%20viability%20and%20metastasis%20of%20the%20cancer%20cells.%22%2C%22date%22%3A%22August%2015%2C%202020%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.lfs.2020.118291%22%2C%22ISSN%22%3A%220024-3205%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0024320520310432%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-08-19T18%3A27%3A40Z%22%7D%7D%5D%7D
Kumari, N., Bharagava, A., & Rath, S. N. (2020). T-type calcium channel antagonist, TTA-A2 exhibits anti-cancer properties in 3D spheroids of A549, a lung adenocarcinoma cell line. Life Sciences, 118291. https://doi.org/10.1016/j.lfs.2020.118291 Cite