Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsIn three dimensional organotypic assays, peptide-exposed telomerase immortalised fibroblasts and primary cancer-associated fibroblasts from breast carcinoma patients both exhibited a markedly reduced pro-invasive ability of either HT1080 fibrosarcoma or MDA-MB-231 mammary carcinoma cells, respectively.
[Cancers]
6807162 55IJ9BRS items 1 apa 0 default asc 1 153390 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-cc4b99b470053ec68ce5c5097318ec50%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%2255IJ9BRS%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Belli%20et%20al.%22%2C%22parsedDate%22%3A%222020-09%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBelli%2C%20S.%2C%20Franco%2C%20P.%2C%20Iommelli%2C%20F.%2C%20De%20Vincenzo%2C%20A.%2C%20Brancaccio%2C%20D.%2C%20Telesca%2C%20M.%2C%20Merlino%2C%20F.%2C%20Novellino%2C%20E.%2C%20Ranson%2C%20M.%2C%20Del%20Vecchio%2C%20S.%2C%20Grieco%2C%20P.%2C%20Carotenuto%2C%20A.%2C%20%26amp%3B%20Stoppelli%2C%20M.%20P.%20%282020%29.%20Breast%20Tumor%20Cell%20Invasion%20and%20Pro-Invasive%20Activity%20of%20Cancer-Associated%20Fibroblasts%20Co-Targeted%20by%20Novel%20Urokinase-Derived%20Decapeptides.%20%3Ci%3ECancers%3C%5C%2Fi%3E%2C%20%3Ci%3E12%3C%5C%2Fi%3E%289%29%2C%202404.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcancers12092404%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcancers12092404%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3D55IJ9BRS%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Breast%20Tumor%20Cell%20Invasion%20and%20Pro-Invasive%20Activity%20of%20Cancer-Associated%20Fibroblasts%20Co-Targeted%20by%20Novel%20Urokinase-Derived%20Decapeptides%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefania%22%2C%22lastName%22%3A%22Belli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paola%22%2C%22lastName%22%3A%22Franco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francesca%22%2C%22lastName%22%3A%22Iommelli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%22%2C%22lastName%22%3A%22De%20Vincenzo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Diego%22%2C%22lastName%22%3A%22Brancaccio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marialucia%22%2C%22lastName%22%3A%22Telesca%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francesco%22%2C%22lastName%22%3A%22Merlino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ettore%22%2C%22lastName%22%3A%22Novellino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie%22%2C%22lastName%22%3A%22Ranson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Silvana%22%2C%22lastName%22%3A%22Del%20Vecchio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paolo%22%2C%22lastName%22%3A%22Grieco%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alfonso%22%2C%22lastName%22%3A%22Carotenuto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20Patrizia%22%2C%22lastName%22%3A%22Stoppelli%22%7D%5D%2C%22abstractNote%22%3A%22Among%20peritumoral%20cells%2C%20cancer-associated%20fibroblasts%20%28CAFs%29%20are%20major%20facilitators%20of%20tumor%20progression.%20This%20study%20describes%20the%20effects%20of%20two%20urokinase-derived%2C%20novel%20decapeptides%2C%20denoted%20as%20Pep%201%20and%20its%20cyclic%20derivative%20Pep%202.%20In%20a%20mouse%20model%20of%20tumor%20dissemination%2C%20using%20HT1080%20fibrosarcoma%20cells%2C%20Pep%202%20reduced%20the%20number%20and%20size%20of%20lung%20metastases.%20Specific%20binding%20of%20fluoresceinated%20Pep%202%20to%20HT1080%20and%20telomerase%20immortalised%20fibroblasts%20%28TIF%29%20cell%20surfaces%20was%20enhanced%20by%20%26alpha%3Bv%20overexpression%20or%20abolished%20by%20excess%20vitronectin%2C%20anti-%26alpha%3Bv%20antibodies%20or%20silencing%20of%20ITGAV%20%26alpha%3Bv%20gene%2C%20identifying%20%26alpha%3Bv-integrin%20as%20the%20Pep%202%20molecular%20target.%20In%203D-organotypic%20assays%2C%20peptide-exposed%20TIFs%20and%20primary%20CAFs%20from%20breast%20carcinoma%20patients%20both%20exhibited%20a%20markedly%20reduced%20pro-invasive%20ability%20of%20either%20HT1080%20fibrosarcoma%20or%20MDA-MB-231%20mammary%20carcinoma%20cells%2C%20respectively.%20Furthermore%2C%20TIFs%2C%20either%20exposed%20to%20Pep%202%2C%20or%20silenced%20for%20%26alpha%3Bv%20integrin%2C%20were%20impaired%20in%20their%20ability%20to%20chemoattract%20cancer%20cells%20and%20to%20contract%20collagen%20matrices%2C%20exhibiting%20reduced%20%26alpha%3B-smooth%20muscle%20actin%20%28%26alpha%3B-SMA%29%20levels.%20Finally%2C%20peptide%20exposure%20of%20%26alpha%3Bv-expressing%20primary%20CAFs%20led%20to%20the%20downregulation%20of%20%26alpha%3B-SMA%20protein%20and%20to%20a%20dramatic%20reduction%20of%20their%20pro-invasive%20capability.%20In%20conclusion%2C%20the%20ability%20of%20the%20novel%20decapeptides%20to%20interfere%20with%20tumor%20cell%20invasion%20directly%20and%20through%20the%20down-modulation%20of%20CAF%20phenotype%20suggests%20their%20use%20as%20lead%20compounds%20for%20co-targeting%20anti-cancer%20strategies.%22%2C%22date%22%3A%222020%5C%2F9%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fcancers12092404%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F2072-6694%5C%2F12%5C%2F9%5C%2F2404%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-08-27T17%3A12%3A17Z%22%7D%7D%5D%7D
Belli, S., Franco, P., Iommelli, F., De Vincenzo, A., Brancaccio, D., Telesca, M., Merlino, F., Novellino, E., Ranson, M., Del Vecchio, S., Grieco, P., Carotenuto, A., & Stoppelli, M. P. (2020). Breast Tumor Cell Invasion and Pro-Invasive Activity of Cancer-Associated Fibroblasts Co-Targeted by Novel Urokinase-Derived Decapeptides. Cancers, 12(9), 2404. https://doi.org/10.3390/cancers12092404 Cite
