Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsWhen grown under the same stem cell enriching conditions, SHH subgroup medulloblastoma cell lines established tight, highly reproducible 3D spheroids that could be maintained for weeks in culture and formed pathophysiological oxygen gradients.
[Scientific Reports]
6807162 LABWI6F8 items 1 apa 0 default asc 1 159277 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-fc62fac5eef636d9c69a49e644de4cdd%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22LABWI6F8%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Roper%20et%20al.%22%2C%22parsedDate%22%3A%222021-02-19%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERoper%2C%20S.%20J.%2C%20Linke%2C%20F.%2C%20Scotting%2C%20P.%20J.%2C%20%26amp%3B%20Coyle%2C%20B.%20%282021%29.%203D%20spheroid%20models%20of%20paediatric%20SHH%20medulloblastoma%20mimic%20tumour%20biology%2C%20drug%20response%20and%20metastatic%20dissemination.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%204259.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-83809-6%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-83809-6%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3DLABWI6F8%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%223D%20spheroid%20models%20of%20paediatric%20SHH%20medulloblastoma%20mimic%20tumour%20biology%2C%20drug%20response%20and%20metastatic%20dissemination%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%20J.%22%2C%22lastName%22%3A%22Roper%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Franziska%22%2C%22lastName%22%3A%22Linke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%20J.%22%2C%22lastName%22%3A%22Scotting%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beth%22%2C%22lastName%22%3A%22Coyle%22%7D%5D%2C%22abstractNote%22%3A%22Studying%20medulloblastoma%2C%20the%20most%20common%20malignant%20paediatric%20brain%20tumour%2C%20requires%20simple%20yet%20realistic%20in%20vitro%20models.%20In%20this%20study%2C%20we%20optimised%20a%20robust%2C%20reliable%2C%20three-dimensional%20%283D%29%20culture%20method%20for%20medulloblastoma%20able%20to%20recapitulate%20the%20spatial%20conformation%2C%20cell%5Cu2013cell%20and%20cell%5Cu2013matrix%20interactions%20that%20exist%20in%20vivo%20and%20in%20patient%20tumours.%20We%20show%20that%2C%20when%20grown%20under%20the%20same%20stem%20cell%20enriching%20conditions%2C%20SHH%20subgroup%20medulloblastoma%20cell%20lines%20established%20tight%2C%20highly%20reproducible%203D%20spheroids%20that%20could%20be%20maintained%20for%20weeks%20in%20culture%20and%20formed%20pathophysiological%20oxygen%20gradients.%203D%20spheroid%20culture%20also%20increased%20resistance%20to%20standard-of-care%20chemotherapeutic%20drugs%20compared%20to%202D%20monolayer%20culture.%20We%20exemplify%20how%20this%20model%20can%20enhance%20in%20vitro%20therapeutic%20screening%20approaches%20through%20dual-inhibitor%20studies%20and%20continual%20monitoring%20of%20drug%20response.%20Next%2C%20we%20investigated%20the%20initial%20stages%20of%20metastatic%20dissemination%20using%20brain-specific%20hyaluronan%20hydrogel%20matrices.%20RNA%20sequencing%20revealed%20downregulation%20of%20cell%20cycle%20genes%20and%20upregulation%20of%20cell%20movement%20genes%20and%20key%20fibronectin%20interactions%20in%20migrating%20cells.%20Analyses%20of%20these%20upregulated%20genes%20in%20patients%20showed%20that%20their%20expression%20correlated%20with%20early%20relapse%20and%20overall%20poor%20prognosis.%20Our%203D%20spheroid%20model%20is%20a%20significant%20improvement%20over%20current%20in%20vitro%20techniques%2C%20providing%20the%20medulloblastoma%20research%20community%20with%20a%20well-characterised%20and%20functionally%20relevant%20culture%20method.%22%2C%22date%22%3A%222021-02-19%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-83809-6%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-83809-6%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-02-19T23%3A18%3A20Z%22%7D%7D%5D%7D
Roper, S. J., Linke, F., Scotting, P. J., & Coyle, B. (2021). 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination. Scientific Reports, 11(1), 4259. https://doi.org/10.1038/s41598-021-83809-6 Cite
