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Pulmonary Cell NewsScientists describe a detailed protocol for establishing tubuloid cultures from tissue and urine, as well as for generating and characterizing tubuloid cell–derived 3D tubular structures in a perfused microfluidic multi-chip platform.
[Nature Protocols]
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Gijzen, L., Yousef Yengej, F. A., Schutgens, F., Vormann, M. K., Ammerlaan, C. M. E., Nicolas, A., Kurek, D., Vulto, P., Rookmaaker, M. B., Lanz, H. L., Verhaar, M. C., & Clevers, H. (2021). Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip. Nature Protocols, 1–28. https://doi.org/10.1038/s41596-020-00479-w Cite
