Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsResearchers showed that in mouse hippocampal neurons, dense core vesicles fusion was strongly and equally reduced in synaptotagmin-1 (Syt1)- or Syt7-deficient neurons, but combined Syt1/Syt7 deficiency did not reduce fusion further.
[Proceedings of the National Academy of Sciences of the United States of America]
6807162 58KJ3DHX items 1 apa 0 default asc 1 161635 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-17cbcf052e79dbbb53abd9aa2d1032c8%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%2258KJ3DHX%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Westen%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-04%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWesten%2C%20R.%20van%2C%20Poppinga%2C%20J.%2C%20Arazola%2C%20R.%20D.%2C%20Toonen%2C%20R.%20F.%2C%20%26amp%3B%20Verhage%2C%20M.%20%282021%29.%20Neuromodulator%20release%20in%20neurons%20requires%20two%20functionally%20redundant%20calcium%20sensors.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%3C%5C%2Fi%3E%2C%20%3Ci%3E118%3C%5C%2Fi%3E%2818%29.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2012137118%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2012137118%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3D58KJ3DHX%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Neuromodulator%20release%20in%20neurons%20requires%20two%20functionally%20redundant%20calcium%20sensors%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rhod%5Cu00e9%20van%22%2C%22lastName%22%3A%22Westen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Josse%22%2C%22lastName%22%3A%22Poppinga%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Roc%5Cu00edo%20D%5Cu00edez%22%2C%22lastName%22%3A%22Arazola%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ruud%20F.%22%2C%22lastName%22%3A%22Toonen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthijs%22%2C%22lastName%22%3A%22Verhage%22%7D%5D%2C%22abstractNote%22%3A%22Neuropeptides%20and%20neurotrophic%20factors%20secreted%20from%20dense%20core%20vesicles%20%28DCVs%29%20control%20many%20brain%20functions%2C%20but%20the%20calcium%20sensors%20that%20trigger%20their%20secretion%20remain%20unknown.%20Here%2C%20we%20show%20that%20in%20mouse%20hippocampal%20neurons%2C%20DCV%20fusion%20is%20strongly%20and%20equally%20reduced%20in%20synaptotagmin-1%20%28Syt1%29-%20or%20Syt7-deficient%20neurons%2C%20but%20combined%20Syt1%5C%2FSyt7%20deficiency%20did%20not%20reduce%20fusion%20further.%20Cross-rescue%2C%20expression%20of%20Syt1%20in%20Syt7-deficient%20neurons%2C%20or%20vice%20versa%2C%20completely%20restored%20fusion.%20Hence%2C%20both%20sensors%20are%20rate%20limiting%2C%20operating%20in%20a%20single%20pathway.%20Overexpression%20of%20either%20sensor%20in%20wild-type%20neurons%20confirmed%20this%20and%20increased%20fusion.%20Syt1%20traveled%20with%20DCVs%20and%20was%20present%20on%20fusing%20DCVs%2C%20but%20Syt7%20supported%20fusion%20largely%20from%20other%20locations.%20Finally%2C%20the%20duration%20of%20single%20DCV%20fusion%20events%20was%20reduced%20in%20Syt1-deficient%20but%20not%20Syt7-deficient%20neurons.%20In%20conclusion%2C%20two%20functionally%20redundant%20calcium%20sensors%20drive%20neuromodulator%20secretion%20in%20an%20expression-dependent%20manner.%20In%20addition%2C%20Syt1%20has%20a%20unique%20role%20in%20regulating%20fusion%20pore%20duration.%5CnAuthor%20Information%5CnRhod%5Cu00e9%20van%20Westena%2Cb%2C%20Josse%20Poppingaa%2C%20Roc%5Cu00edo%20D%5Cu00edez%20Arazolaa%2C%20Ruud%20F.%20Toonena%2C1%2C%20and%20Matthijs%20Verhagea%2Cb%2C1aDepartment%20of%20Functional%20Genomics%2C%20Center%20for%20Neurogenomics%20and%20Cognitive%20Research%2C%20Neuroscience%20Campus%20Amsterdam%2C%20Vrije%20Universiteit%20Amsterdam%2C%201081%20HV%20Amsterdam%2C%20The%20Netherlands%3BbDepartment%20of%20Clinical%20Genetics%2C%20Amsterdam%20University%20Medical%20Centers%2C%201081%20HV%20Amsterdam%2C%20The%20NetherlandsEdited%20by%20Wade%20G.%20Regehr%2C%20Harvard%20Medical%20School%2C%20Boston%2C%20MA%2C%20and%20accepted%20by%20Editorial%20Board%20Member%20David%20E.%20Clapham%20March%203%2C%202021%20%28received%20for%20review%20June%2022%2C%202020%29%5CnFootnotes%5Cu21b51To%20whom%20correspondence%20may%20be%20addressed.%20Email%3A%20r.f.g.toonen%40vu.nl%20or%20matthijs%40cncr.vu.nl.Author%20contributions%3A%20R.v.W.%2C%20R.F.T.%2C%20and%20M.V.%20designed%20research%3B%20R.v.W.%20and%20J.P.%20performed%20research%3B%20R.v.W.%20and%20R.D.A.%20contributed%20new%20reagents%5C%2Fanalytic%20tools%3B%20R.v.W.%20and%20J.P.%20analyzed%20data%3B%20and%20R.v.W.%2C%20R.F.T.%2C%20and%20M.V.%20wrote%20the%20paper.The%20authors%20declare%20no%20competing%20interest.This%20article%20is%20a%20PNAS%20Direct%20Submission.%20W.G.R.%20is%20a%20guest%20editor%20invited%20by%20the%20Editorial%20Board.This%20article%20contains%20supporting%20information%20online%20at%20https%3A%5C%2F%5C%2Fwww.pnas.org%5C%2Flookup%5C%2Fsuppl%5C%2Fdoi%3A10.1073%5C%2Fpnas.2012137118%5C%2F-%5C%2FDCSupplemental.%22%2C%22date%22%3A%222021%5C%2F05%5C%2F04%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.2012137118%22%2C%22ISSN%22%3A%220027-8424%2C%201091-6490%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.pnas.org%5C%2Fcontent%5C%2F118%5C%2F18%5C%2Fe2012137118%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-28T16%3A49%3A00Z%22%7D%7D%5D%7D
Westen, R. van, Poppinga, J., Arazola, R. D., Toonen, R. F., & Verhage, M. (2021). Neuromodulator release in neurons requires two functionally redundant calcium sensors. Proceedings of the National Academy of Sciences, 118(18). https://doi.org/10.1073/pnas.2012137118 Cite
