Researchers used phasor – Fluorescence Lifetime Imaging Microscopy to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation.
[Communications Biology]
7753456 {7753456:ACBT2H9Z} apa 50 1 162572 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-0c960f3492679b9cc3635235bdd584d3%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22ACBT2H9Z%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Wang%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-19%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWang%2C%20Z.%2C%20Gurlo%2C%20T.%2C%20Matveyenko%2C%20A.%20V.%2C%20Elashoff%2C%20D.%2C%20Wang%2C%20P.%2C%20Rosenberger%2C%20M.%2C%20Junge%2C%20J.%20A.%2C%20Stevens%2C%20R.%20C.%2C%20White%2C%20K.%20L.%2C%20Fraser%2C%20S.%20E.%2C%20%26amp%3B%20Butler%2C%20P.%20C.%20%282021%29.%20Live-cell%20imaging%20of%20glucose-induced%20metabolic%20coupling%20of%20%26%23x3B2%3B%20and%20%26%23x3B1%3B%20cell%20metabolism%20in%20health%20and%20type%202%20diabetes.%20%3Ci%3ECommunications%20Biology%3C%5C%2Fi%3E%2C%20%3Ci%3E4%3C%5C%2Fi%3E%281%29%2C%201%26%23x2013%3B11.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs42003-021-02113-1%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs42003-021-02113-1%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3DACBT2H9Z%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Live-cell%20imaging%20of%20glucose-induced%20metabolic%20coupling%20of%20%5Cu03b2%20and%20%5Cu03b1%20cell%20metabolism%20in%20health%20and%20type%202%20diabetes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhongying%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tatyana%22%2C%22lastName%22%3A%22Gurlo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aleksey%20V.%22%2C%22lastName%22%3A%22Matveyenko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Elashoff%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peiyu%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Madeline%22%2C%22lastName%22%3A%22Rosenberger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jason%20A.%22%2C%22lastName%22%3A%22Junge%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Raymond%20C.%22%2C%22lastName%22%3A%22Stevens%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kate%20L.%22%2C%22lastName%22%3A%22White%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Scott%20E.%22%2C%22lastName%22%3A%22Fraser%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%20C.%22%2C%22lastName%22%3A%22Butler%22%7D%5D%2C%22abstractNote%22%3A%22Type%202%20diabetes%20is%20characterized%20by%20%5Cu03b2%20and%20%5Cu03b1%5Cu00a0cell%20dysfunction.%20We%20used%20phasor-FLIM%20%28Fluorescence%20Lifetime%20Imaging%20Microscopy%29%20to%20monitor%20oxidative%20phosphorylation%20and%20glycolysis%20in%20living%20islet%20cells%20before%20and%20after%20glucose%20stimulation.%20In%20healthy%20cells%2C%20glucose%20enhanced%20oxidative%20phosphorylation%20in%20%5Cu03b2%5Cu00a0cells%20and%20suppressed%20oxidative%20phosphorylation%20in%20%5Cu03b1%5Cu00a0cells.%20In%20Type%202%20diabetes%2C%20glucose%20increased%20glycolysis%20in%20%5Cu03b2%5Cu00a0cells%2C%20and%20only%20partially%20suppressed%20oxidative%20phosphorylation%20in%20%5Cu03b1%5Cu00a0cells.%20FLIM%20uncovers%20key%20perturbations%20in%20glucose%20induced%20metabolism%20in%20living%20islet%20cells%20and%20provides%20a%20sensitive%20tool%20for%20drug%20discovery%20in%20diabetes.%22%2C%22date%22%3A%222021-05-19%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs42003-021-02113-1%22%2C%22ISSN%22%3A%222399-3642%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs42003-021-02113-1%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%223XSKJF5U%22%5D%2C%22dateModified%22%3A%222021-05-19T20%3A26%3A46Z%22%7D%7D%5D%7D
Wang, Z., Gurlo, T., Matveyenko, A. V., Elashoff, D., Wang, P., Rosenberger, M., Junge, J. A., Stevens, R. C., White, K. L., Fraser, S. E., & Butler, P. C. (2021). Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes. Communications Biology, 4(1), 1–11. https://doi.org/10.1038/s42003-021-02113-1 Cite