Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsUnfolding a Zika virus xrRNA with optical tweezers revealed that it was the most mechanically stable RNA yet observed.
[Nature Chemical Biology]
6445218 N7JZWZCK items 1 apa 0 default asc 1 164470 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-371fe5ec5473f191691c51e9e31b5ddc%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22N7JZWZCK%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zhao%20and%20Woodside%22%2C%22parsedDate%22%3A%222021-07-12%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZhao%2C%20M.%2C%20%26amp%3B%20Woodside%2C%20M.%20T.%20%282021%29.%20Mechanical%20strength%20of%20RNA%20knot%20in%20Zika%20virus%20protects%20against%20cellular%20defenses.%20%3Ci%3ENature%20Chemical%20Biology%3C%5C%2Fi%3E%2C%201%26%23x2013%3B7.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41589-021-00829-z%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41589-021-00829-z%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DN7JZWZCK%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mechanical%20strength%20of%20RNA%20knot%20in%20Zika%20virus%20protects%20against%20cellular%20defenses%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Meng%22%2C%22lastName%22%3A%22Zhao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20T.%22%2C%22lastName%22%3A%22Woodside%22%7D%5D%2C%22abstractNote%22%3A%22Unusual%20knot-like%20structures%20recently%20discovered%20in%20viral%20exoribonuclease-resistant%20RNAs%20%28xrRNAs%29%20prevent%20digestion%20by%20host%20RNases%20to%20create%20subgenomic%20RNAs%20enhancing%20infection%20and%20pathogenicity.%20xrRNAs%20are%20proposed%20to%20prevent%20digestion%20through%20mechanical%20resistance%20to%20unfolding.%20However%2C%20their%20unfolding%20force%20has%20not%20been%20measured%2C%20and%20the%20factors%20determining%20RNase%20resistance%20are%20unclear.%20Furthermore%2C%20how%20these%20knots%20fold%20remains%20unknown.%20Unfolding%20a%20Zika%20virus%20xrRNA%20with%20optical%20tweezers%20revealed%20that%20it%20was%20the%20most%20mechanically%20stable%20RNA%20yet%20observed.%20The%20knot%20formed%20by%20threading%20the%205%5Cu2032%20end%20into%20a%20three-helix%20junction%20before%20pseudoknot%20interactions%20closed%20a%20ring%20around%20it.%20The%20pseudoknot%20and%20tertiary%20contacts%20stabilizing%20the%20threaded%205%5Cu2032%20end%20were%20both%20required%20to%20generate%20extreme%20force%20resistance%2C%20whereas%20removing%20a%205%5Cu2032-end%20contact%20produced%20a%20low-force%20knot%20lacking%20RNase%20resistance.%20These%20results%20indicate%20mechanical%20resistance%20plays%20a%20central%20functional%20role%2C%20with%20the%20fraction%20of%20molecules%20forming%20extremely%20high-force%20knots%20determining%20the%20RNase%20resistance%20level.%22%2C%22date%22%3A%222021-07-12%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41589-021-00829-z%22%2C%22ISSN%22%3A%221552-4469%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41589-021-00829-z%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-07-12T21%3A22%3A57Z%22%7D%7D%5D%7D
Zhao, M., & Woodside, M. T. (2021). Mechanical strength of RNA knot in Zika virus protects against cellular defenses. Nature Chemical Biology, 1–7. https://doi.org/10.1038/s41589-021-00829-z Cite
