CRISPR/Cas9/AAV9-Mediated In Vivo Editing Identifies MYC Regulation of 3D Genome in Skeletal Muscle Stem Cell

Researchers demonstrated efficient editing of master transcription factor (TF)Myod1 locus using the CRISPR/Cas9/AAV9-sgRNA system in juvenile satellite cells (SCs). Application on two key TFs, MYC and BCL6, unveiled distinct functions in SC activation and muscle regeneration.