The Onset of Circulation Triggers a Metabolic Switch Required for Endothelial to Hematopoietic Transition

Investigators showed that in Ncx1−/− mouse embryos devoid of circulation, the HSC lineage developed until the phenotypic pro-HSC stage. Experimental activation of glycolysis resulted in decreased intraembryonic hematopoiesis, suggesting that the onset of circulation triggered metabolic changes that allowed HSC generation to proceed.