Dynamic nuclear polarization‐magnetic resonance imaging chemical shift imaging with hyperpolarized [1‐13C] pyruvate was conducted to evaluate the metabolic change in glycolytic profiles after radiation of two glioma stem‐like cell‐derived gliomas and an adherent human glioblastoma cell line in an orthotopic xenograft mouse model.
[Nmr in Biomedicine]
6807162 {6807162:VSQW9KWD} apa 50 1 161892 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-f7af1b7a2fb83707134e9b1f82a3b900%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22VSQW9KWD%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kawai%20et%20al.%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKawai%2C%20T.%2C%20Brender%2C%20J.%20R.%2C%20Lee%2C%20J.%20A.%2C%20Kramp%2C%20T.%2C%20Kishimoto%2C%20S.%2C%20Krishna%2C%20M.%20C.%2C%20Tofilon%2C%20P.%2C%20%26amp%3B%20Camphausen%2C%20K.%20A.%20%28n.d.%29.%20Detection%20of%20metabolic%20change%20in%20glioblastoma%20cells%20after%20radiotherapy%20using%20hyperpolarized%2013C-MRI.%20%3Ci%3ENMR%20in%20Biomedicine%3C%5C%2Fi%3E%2C%20%3Ci%3En%5C%2Fa%3C%5C%2Fi%3E%28n%5C%2Fa%29%2C%20e4514.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2Fhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fnbm.4514%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2Fhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fnbm.4514%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3DVSQW9KWD%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Detection%20of%20metabolic%20change%20in%20glioblastoma%20cells%20after%20radiotherapy%20using%20hyperpolarized%2013C-MRI%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tatsuya%22%2C%22lastName%22%3A%22Kawai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeffrey%20R.%22%2C%22lastName%22%3A%22Brender%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%20A.%22%2C%22lastName%22%3A%22Lee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tamalee%22%2C%22lastName%22%3A%22Kramp%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shun%22%2C%22lastName%22%3A%22Kishimoto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Murali%20C.%22%2C%22lastName%22%3A%22Krishna%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philip%22%2C%22lastName%22%3A%22Tofilon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kevin%20A.%22%2C%22lastName%22%3A%22Camphausen%22%7D%5D%2C%22abstractNote%22%3A%22Dynamic%20nuclear%20polarization%20%28DNP%29%20of%2013C-labeled%20substrates%20enables%20the%20use%20of%20magnetic%20resonance%20imaging%20%28MRI%29%20to%20monitor%20specific%20enzymatic%20reactions%20in%20tumors%20and%20offers%20an%20opportunity%20to%20investigate%20these%20differences.%20In%20this%20study%2C%20DNP-MRI%20chemical%20shift%20imaging%20with%20hyperpolarized%20%5B1-13C%5D%20pyruvate%20was%20conducted%20to%20evaluate%20the%20metabolic%20change%20in%20glycolytic%20profiles%20after%20radiation%20of%20two%20glioma%20stem-like%20cell-derived%20gliomas%20%28GBMJ1%20and%20NSC11%29%20and%20an%20adherent%20human%20glioblastoma%20cell%20line%20%28U251%29%20in%20an%20orthotopic%20xenograft%20mouse%20model.%20The%20DNP-MRI%20showed%20an%20increase%20in%20Lac%5C%2FPyr%20at%206%20and%2016%20h%20after%20irradiation%20%2818%25%20%5Cu00b1%204%25%20and%2014%25%20%5Cu00b1%203%25%2C%20respectively%3B%20mean%20%5Cu00b1%20SEM%29%20compared%20with%20unirradiated%20controls%20in%20GBMJ1%20tumors%2C%20whereas%20no%20significant%20change%20was%20observed%20in%20U251%20and%20NSC11%20tumors.%20Metabolomic%20analysis%20likewise%20showed%20a%20significant%20increase%20in%20lactate%20in%20GBMJ1%20tumors%20at%2016%20h.%20An%20immunoblot%20assay%20showed%20upregulation%20of%20lactate%20dehydrogenase-A%20expression%20in%20GBMJ1%20following%20radiation%20exposure%2C%20consistent%20with%20DNP-MRI%20and%20metabolomic%20analysis.%20In%20conclusion%2C%20our%20preclinical%20study%20demonstrates%20that%20the%20DNP-MRI%20technique%20has%20the%20potential%20to%20be%20a%20powerful%20diagnostic%20method%20with%20which%20to%20evaluate%20GBM%20tumor%20metabolism%20before%20and%20after%20radiation%20in%20the%20clinical%20setting.%22%2C%22date%22%3A%22%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fnbm.4514%22%2C%22ISSN%22%3A%221099-1492%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fanalyticalsciencejournals.onlinelibrary.wiley.com%5C%2Fdoi%5C%2Fabs%5C%2F10.1002%5C%2Fnbm.4514%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-05-04T20%3A01%3A29Z%22%7D%7D%5D%7D
Kawai, T., Brender, J. R., Lee, J. A., Kramp, T., Kishimoto, S., Krishna, M. C., Tofilon, P., & Camphausen, K. A. (n.d.). Detection of metabolic change in glioblastoma cells after radiotherapy using hyperpolarized 13C-MRI. NMR in Biomedicine, n/a(n/a), e4514. https://doi.org/https://doi.org/10.1002/nbm.4514 Cite