Researchers evaluate elongating multi-lineage organized over a 40 day period, applying immunofluorescence of multi-lineage and functional biomarkers, including day 16 single-cell RNA-Seq, and evaluation of ectodermal and non-ectodermal neural crest cells.
[Nature Communications]
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Olmsted, Z. T., & Paluh, J. L. (2021). Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids. Nature Communications, 12(1), 3020. https://doi.org/10.1038/s41467-021-23294-7 Cite