PTEN

[Cell Death & Disease] Researchers probed the function and potential mechanism of KCNK15-AS1 in pancreatic cancer (PC) cell growth. RT-qPCR and western blot were employed to measure gene expression in PC cells.

[Inflammation Research] miR-342-3p overexpression in LCSC lead to lower tumor volume, reduced tumor spheroid formation and agar colony formation rates, as well as lower mRNA and protein expressions of CD44, ALDH1, Bmi1, Sox2, and Oct4.

[Molecular Therapy-Nucleic Acids] RNA sequencing, qRT-PCR, and Western blot analysis were used to identify the aberrant expression and signaling of miR-148a-3p within cells, mice, and patients with Alzheimer’s disease (AD). Researchers implicated the miR-148a-3p/p35/PTEN pathway as an essential contributor to tau hyperphosphorylation and feed-forward regulation in AD.

[International Journal of Molecular Sciences] The expression of ALDH1B1-regulated genes, including PTEN, CTNNB1, and CSC-related β-catenin target genes, was inversely regulated by AMBRA1, suggesting a negative regulatory role of AMBRA1 in the expression of ALDH1B1-regulated genes.

[Cell Communication and Signaling] A dystrophin-deficient myoblast cell line established from the skeletal muscle of a dystrophic mouse was used as a model. The dfd13 and C2C12 myoblasts were cultured in low mitogen conditions for ten days to induce differentiation.

[Stem Cell Reports] While hematopoietic-specific Cited2 deletion has a minor impact on steady-state hematopoiesis, Cited2-deficient hematopoietic stem cells are severely depleted in young mice and fail to expand upon aging.