CRISPR-Cas9

[Scientific Reports] Researchers optimized an homology-directed repair (HDR)-mediated knock-in method for mouse ESCs by improving the efficiency of HDR-mediated knock-in of a plasmid donor while almost completely suppressing non-homologous end joining/microhomology-mediated end joining-based integration.

[American Journal of Gastrointestinal and Liver Physiology] Investigators analyzed the autophagy process in high MUC2-producing human HT29-H and a clone HT29-L silenced for MUC2 expression by lentivirus-mediated shRNA, and wild-type and CRISPR/Cas9 MUC2 KO LS174T cells.

[EdiGene, Inc. (Business Wire, Inc.)] EdiGene, Inc. announced the first patient enrolled in multicenter Phase I clinical study of its investigational gene-editing hematopoietic stem cell therapy ET-01 for patients with transfusion dependent β-thalassemia at the Institute of Hematology and Blood Diseases Hospital.

[Archives of Toxicology] Researchers presented the generation and application of a fluorescent human iPSC reporter line for heme oxygenase-1, which is considered a sensitive and reliable biomarker for the oxidative stress response.

[Science Advances] Researchers used CRISPR-Cas9 genome editing of iPSCs to create a synthetic gene circuit that sensed changing levels of endogenous inflammatory cytokines to trigger a proportional therapeutic response.

[EMBO Journal] Scientists reported that the single nucleotide polymorphism (SNP) rs4971059, one of 65 new breast cancer risk loci identified in a recent genome-wide association study, functioned as an active enhancer of TRIM46 expression. TRIM46 promoted breast cancer cell proliferation and chemoresistance in vitro and accelerated tumor growth in vivo.