CRISPR-Cas9

[Cell Reports] Via genome-scale CRISPR-Cas9 screening, scientists identified protein arginine methyltransferase 1 (PRMT1) as a critical mediator of androgen receptor expression and signaling.

[Nature Communications] Scientists identified by snATAC-seq a 42 kb super-enhancer at the locus regrouping the fast Myosin genes.

[Molecular Psychiatry] Investigators used hiPSCs along with CRISPR-Cas9 gene editing to investigate the contribution of chromosome 21 candidate genes to Alzheimer’s disease-relevant neuronal phenotypes.

[Nature Communications] Scientists found that Dicer-deficient ESCs had strong proliferation and chromosome segregation defects as well as increased transcription of centromeric satellite repeats, which triggered the interferon response.

[Scientific Reports] Utilizing dual-single guide RNAs (sgRNAs) and a homology-directed repair template with the CRISPR/Cas9 gene-editing system is a promising avenue for the treatment of glioblastoma multiforme.

[Blood] Using knock-down by shRNA and CRISPR/dCas9, scientists demonstrated that expression of SEMA4A was essential for normal myeloma cell growth in vitro, indicating that myeloma cells cannot downregulate the protein to avoid detection.