CRISPR-Cas9

[Biophysical Journal] Scientists developed a homozygous NaV1.5 KO iPSC line able to differentiate into cardiomyocytes (iPSC-CMs) with CRISPR/Cas9 tool. The NaV1.5 KO iPSC-CMs exhibited an organized contractile apparatus, spontaneous contractile activity, and electrophysiological recordings confirmed the major reduction in total Na+ currents.

[Cell Death & Disease] Knockout of diacylglycerol kinase ζ (DGKZ) in TNBC cell lines were found to significantly inhibit metastatic behaviors in vitro and in vivo, whereas its overexpression increased the metastatic potential of cell lines.

[Brain] To model human malformations of cortical development associated with nitrogen permease regulator-like 3 (NPRL3) variants, researchers created a focal Nprl3 KO in fetal mouse cortex by in utero electroporation and found altered cortical lamination and white matter heterotopic neurons

[Haematologica] Researchers developed a CD38 CAR-NK cell therapy for acute myeloid leukemia, first by using an NK cell line which had low baseline CD38 expression and subsequently NK cells expanded from healthy donors.

[Stem Cell Reports] The authors used Duchenne muscular dystrophy-patient-specific human iPSCs to model cardiomyopathic features and unravel novel pathologic insights.

[Frontiers in Molecular Neuroscience] Researchers described the use of an MSC-based delivery system for the secretion of a Zinc fingers protein in transgenic mouse models and young rhesus monkeys.