gene knockdown

[Cell Stem Cell] Scientists profiled scarring versus YAP-inhibition-induced wound regeneration at the transcriptional, protein, and tissue levels, and showed that disrupting YAP mechanotransduction yielded regenerative repair by fibroblasts with activated Trps1 and Wnt signaling.

[Oxidative Medicine and Cellular Longevity] The authors investigated the effects of silencing salusin-β on endothelial function, cardiac function, vascular and myocardial remodeling, and its underlying signaling pathways in chronic heart failure rats induced by coronary artery ligation.

[Journal of Experimental & Clinical Cancer Research] RSL1D1 distributed throughout the nucleus in human colorectal cancer cells. Silencing of RSL1D1 gene induced cell cycle arrest at G1/S and cell apoptosis in a p53-dependent manner.

[Cell Death & Disease] Gene knockdown of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex, 4-like 2 (NDUFA4L2) inhibited tumor cell proliferation and enhanced apoptosis, while tumor cells initiated protective mitophagy in vitro and in vivo.

[STAR Protocols] Scientists shared their in-house optimized protocols to examine organoid bioenergetics in response to drugs, gene knockdown, or to characterize the metabolism of specific cell types.

[Cell Reports] Scientists showed that in mouse hippocampal synapses, synaptic vesicle (SV) docking initiates at ∼12 nm to the active zone (AZ) by Synaptotagmin-1 (Syt1). They also demonstrated that PI(4,5)P2 was the membrane partner of Syt1 to initiate SV docking, and disrupting their interaction could abolish the docking initiation.