induced pluripotent stem cells

[Nature Biomedical Engineering] Researchers showed that patterned organoids and bioprinted tissues with controlled composition and organization could be generated by simultaneously co-differentiating hiPSCs into distinct cell types via the forced overexpression of transcription factors, independently of culture-media composition.

[Advanced Science] Investigators developed in vitro methods using a kidney decellularized ECM hydrogel to culture human pluripotent stem cell-derived kidney organoids, which had extensive vascular network and their own endothelial cells.

[Cell Stem Cell] Researchers induced efficient human pluripotent stem-cell-derived cardiomyocytes maturation through metabolic-pathway modulations and found that peroxisome-proliferator-associated receptor (PPAR) signaling regulated glycolysis and fatty acid oxidation in an isoform-specific manner.

[Methods] Scientists designed a workflow to reproducibly extract proteins from brain organoids, measure global turnover using mass spectrometry, and statistically investigate turnover differences between genotypes.

[iScience] The authors applied high-dimensional design of experiments methodology to identify critical process parameters that govern neural territory patterning from pluripotency — the first stage toward specification of central nervous system cell fates.

[Advanced Science] An hiPSC-derived epithelial and mesenchymal organoid model in a 3D culture system for localized scleroderma therapy was established.