induced pluripotent stem cells

[Development] Scientists reported an organoid model of neural tube extension derived from human human PSC aggregates that had been caudalized with Wnt agonism, enabling them to recapitulate aspects of the morphological and temporal gene expression patterns of neural tube development.

[NPJ Regenerative Medicine] Scientists summarize the use of different human cell populations for preclinical and clinical tissue-engineered skin substitutes under research, remarking their strengths and limitations and discuss the future perspectives, which could be useful for wound healing purposes.

[Dermatologic Therapy] The authors review literature about ongoing and completed clinical trials that applied fibroblasts and bioengineered fibroblasts as therapeutic agents for various skin disorders.

[Nature Communications] Scientists generated human induced pluripotent stem cells (hiPSCs) carrying a TNNI1EmGFP and TNNI3mCherry double reporter to monitor and isolate mature sub-populations during cardiac differentiation, and showed that ERRγ-treated hiPSC-cardiomyocytes (CMs) had a mature cellular property consistent with neonatal CMs.

[Scientific Reports] The authors employed a robust method, without the need for mouse co-culture to generate 3D cerebellar organoids from human PSCs that displayed hallmarks of in vivo cerebellar development.

[Stem Cell Reports] Researchers developed a rapid protein-level OCT4 depletion system that demonstrated that the immediate downstream response to loss of OCT4 was reduced expression of key pluripotency factors.