organoids

[eLife] Using a human fetal lung organoid system as an example the authors systematically developed and optimized a complete genetic toolbox for use in tissue-derived organoids.

[Molecular Cancer Research] Researchers examined the role of DKK3 by overexpression and knockdown and discovered that DKK3 suppressed Wnt signaling in APC-null murine colonic organoids and human colon cancer cells despite the presence of downstream activating mutations in the Wnt pathway.

[Hepatology International] Genetically modified organoids have been developed by sequential knockout and knockin of driver mutations by genome editing followed by niche-based selection.

[Cellular Oncology] Cell line-derived spheroids, ex vivo intestinal organoid cultures and induced-pluripotent stem cells were used to investigate the role of atypical cyclin P in stemness.

[Nature Communications] Researchers derived pancreatic ductal adenocarcinoma organoids from 18 primary tumors and two matched liver metastases, and showed that ‘classical’ and ‘basal-like’ cells coexist in individual organoids.

[Oncogene] To understand whether transcriptional activity of RING-finger protein 6 (RNF6) underlied its oncogenic effect, the authors performed integrated chromatin immunoprecipitation-sequencing and RNA-sequencing analysis to identify splicing factor 3b subunit 2 (SF3B2) as a potential downstream target of RNF6.