protein expression

[Journal of Cellular and Molecular Medicine] Liver SMAD2 phosphorylation was down-regulated in all series with matrix metalloproteinase (MMP) inhibition or knock-out, and MMP-9 inhibition or deletion ameliorated the severity of cirrhosis, portal hypertension, and associated derangements.

[Cell Death Discovery] Researchers evaluated the effects of Pgam5 on neurological deficits and neuroinflammation of controlled cortical impact-induced TBI mouse model in vivo and LPS+ ATP-induced microglia model in vitro.

[Scientific Reports] Rat primary cardiac fibroblasts were isolated and transformed into myofibroblasts using 5 ng/ml TGF-β1. Transformation of cells to myofibroblasts was confirmed with the presence of α-smooth muscle actin using Western blot.

[Cell Discovery] Liver-specific Sirutin 2 (SIRT2) deficiency sensitized mice to alcoholic liver disease, whereas transgenic SIRT2 overexpression in hepatocytes significantly prevented ethanol-induced liver injury via normalization of hepatic steatosis, lipid peroxidation, and hepatocyte apoptosis.

[iScience] Spheroid cultures of primary human hepatocytes are used in studies of hepatic drug metabolism and toxicity. The authors performed an in-depth analysis of the influence of various culture conditions to find the optimal conditions for the maintenance of an in vivo like phenotype.

[Scientific Reports] Researchers investigated the effect of differential expression of CYP2R1 in hepatocytes on the expression of genes related to liver fibrosis in primary hepatic stellate cells of biliary atresia and animal models of cholestasis.