reporter assay

[Laboratory Investigation] Pulmonary artery smooth muscle cells were cultured in hypoxia condition for constructing a pulmonary arterial hypertension model in vitro. The expression of genes and proteins were determined by qRT-PCR and western bolt assays.

[Journal of Bioscience and Bioengineering] Investigators developed a reporter assay using human iPSCs to detect changes in the fibroblast growth factor signaling pathway, a pathway essential for limb morphogenesis.

[Experimental Cell Research] Flow cytometry and sphere formation assays indicated that LINC01232 significantly promoted non-small cell lung carcinoma cell stemness.

[iScience] Scientists proposed a reporter assay that utilized human iPSCs to detect changes in fibroblast growth factor signaling, which was essential for limb morphogenesis.

[International Journal of Molecular Medicine] The altered expression of microRNAs has been found in asthma. However, the detailed mechanisms responsible for the effects of miR‑375 on Dex‑induced airway epithelial cell dysfunction remain elusive. Researchers elucidated these mechanisms.

[Journal of Nanobiotechnology] Researchers investigated the effects of exosomes derived from bone marrow mesenchymal stem cells on the regulation of acute myeloid leukemia and the underlying mechanisms mediated by microRNA.