Investigators discuss the cellular phenotypes and spatial patterns of the lymphoid-, myeloid-, and stromal cells in the TNBC microenvironment and the potential value of mapping these features onto tumor cell genotypes.
Scientists purified different cochlear cell types from neonatal mice, validated the composition of the different groups with single-cell RNA sequencing, and assessed the various groups’ potential to grow into inner ear organoids.
Deficiency of Vsnl1 not only reduced the beating rate of human iPSC- derived cardiomyocytes but also the heart rate of mice. Weighted gene co-expression network analysis unveiled the core gene regulation network governing the function of the sinoatrial node in mice.
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Liang, D., Xue, J., Geng, L., Zhou, L., Lv, B., Zeng, Q., Xiong, K., Zhou, H., Xie, D., Zhang, F., Liu, J., Liu, Y., Li, L., Yang, J., Xue, Z., & Chen, Y.-H. (2021). Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nature Communications, 12(1), 287. https://doi.org/10.1038/s41467-020-20448-x Cite
Mouse lineage tracing, organoid culture and single-molecule RNA in situ hybridizations revealed that the two epithelia derive from separate cervix-resident lineage-specific stem cell populations regulated by opposing Wnt signals from the stroma.
[Nature Cell Biology]
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Chumduri, C., Gurumurthy, R. K., Berger, H., Dietrich, O., Kumar, N., Koster, S., Brinkmann, V., Hoffmann, K., Drabkina, M., Arampatzi, P., Son, D., Klemm, U., Mollenkopf, H.-J., Herbst, H., Mangler, M., Vogel, J., Saliba, A.-E., & Meyer, T. F. (2021). Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia. Nature Cell Biology, 1–14. https://doi.org/10.1038/s41556-020-00619-0 Cite
Researchers profiled the gene expression of single cells from human hepatocellular carcinoma (HCC) tumor and para-tumor tissues using the Smart-seq 2 sequencing method. Based on differentially expressed genes, they identified heterogeneous subclones in HCC tissues, including five HCC and two hepatocyte subclones.
[Cell Death Discovery]
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Dong, X., Wang, F., Liu, C., Ling, J., Jia, X., Shen, F., Yang, N., Zhu, S., Zhong, L., & Li, Q. (2021). Single-cell analysis reveals the intra-tumor heterogeneity and identifies MLXIPL as a biomarker in the cellular trajectory of hepatocellular carcinoma. Cell Death Discovery, 7(1), 1–13. https://doi.org/10.1038/s41420-021-00403-5 Cite
Researchers demonstrated that altered dendritic cell representation in the skin-draining lymph nodes during sensitization to allergens caused Th2-driven contact hypersensitivity.
[Proceedings of the National Academy of Sciences of the United States of America]
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Miller, H. L., Andhey, P. S., Swiecki, M. K., Rosa, B. A., Zaitsev, K., Villani, A.-C., Mitreva, M., Artyomov, M. N., Gilfillan, S., Cella, M., & Colonna, M. (2021). Altered ratio of dendritic cell subsets in skin-draining lymph nodes promotes Th2-driven contact hypersensitivity. Proceedings of the National Academy of Sciences, 118(3). https://doi.org/10.1073/pnas.2021364118 Cite
The authors discuss how the view of the airway epithelial landscape has evolved with the advent of transcriptomic approaches to cellular phenotyping, with a focus on epithelial interactions with the local neuronal and immune systems.
[Nature Reviews Immunology]
Investigators demonstrated using an ESC model that transient expression of the T-box transcription factor Eomesodermin governs haemogenic competency of extra-embryonic mesoderm.
[Nature Cell Biology]
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Harland, L. T. G., Simon, C. S., Senft, A. D., Costello, I., Greder, L., Imaz-Rosshandler, I., Göttgens, B., Marioni, J. C., Bikoff, E. K., Porcher, C., de Bruijn, M. F. T. R., & Robertson, E. J. (2021). The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nature Cell Biology, 23(1), 61–74. https://doi.org/10.1038/s41556-020-00611-8 Cite
Scientists combined bulk RNA sequencing, single cell RNA sequencing, and bioinformatic analyses, including weighted gene co-expression analysis, to investigate the gene regulatory networks regulating human iPSC differentiation under chondrogenic conditions.
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Scientists investigated freshly isolated, uncultured umbilical cord (UC)-MSCs by single-cell RNA sequencing and found two populations of UC-MSCs.
[Stem Cell Research & Therapy]
Scientists compared African green monkeys inoculated with infectious SARS-CoV-2 or irradiated, inactivated virus to study the dynamics of virus replication throughout the respiratory tract. Interestingly, genomic RNA from the animals inoculated with the irradiated virus was found to be highly stable, whereas sub-genomic RNA, an indicator of viral replication, was found to degrade quickly.
[Science Translational Medicine]
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Speranza, E., Williamson, B. N., Feldmann, F., Sturdevant, G. L., Pérez, L. P., Meade-White, K., Smith, B. J., Lovaglio, J., Martens, C., Munster, V. J., Okumura, A., Shaia, C., Feldmann, H., Best, S. M., & Wit, E. de. (2021). Single-cell RNA sequencing reveals SARS-CoV-2 infection dynamics in lungs of African green monkeys. Science Translational Medicine. https://doi.org/10.1126/scitranslmed.abe8146 Cite