single-cell RNA sequencing

[iScience] Scientists utilized a systems-based approach to compare MSCs from different genetic backgrounds that displayed differences in antibacterial activity.

[Communications Biology] Researchers investigated Parkinson’s disease (PD)'s mechanism using dopaminergic neurons differentiated from human iPSCs carrying the ILE368ASN mutation within the PINK1 gene, which is strongly associated with PD.

[Hepatology] The authors performed a Sleeping Beauty transposon-based in vivo insertional mutagenesis screen in liver-specific Pten-deficient mice and identified tumor necrosis factor receptor-related factor 3 (Traf3) as the most significantly mutated gene in murine intrahepatic cholangiocarcinoma in a loss-of-function manner.

[Journal of Immunology] The authors integrated six bead-enriched T cell subsets with 62,235 single-cell transcriptomes from human PBMCs and clustered them into nine scRNA-seq clustered populations.

[PLoS Pathogens] Both in vitro and in vivo Treg migration was effectively blocked by a novel, small molecule antagonist of CCR4, CCR4-351.

[Science Immunology] Monocyte-derived macrophages contributed to the resident macrophage population until they reached a defined upper limit after which they did not outcompete pre-existing resident macrophages.