single-cell RNA sequencing

[Science Advances] Scientists used a transcriptomics-guided drug target discovery strategy using gene signatures of smoking-associated COPD and from mice chronically exposed to cigarette smoke, identifying druggable targets expressed in alveolar epithelial progenitors, of which they screened the function in lung organoids.

[Journal of Physiology] Single-cell RNA sequencing and functional assays were performed to demonstrate that pericytes in mouse skeletal muscle lost the capacity to synthesize antioxidants during disuse and recovery.

[Nature Biomedical Engineering] The authors showed that druggable biological targets and small-molecule regulators of intestinal stem cell differentiation could be identified via multiplexed phenotypic screening using thousands of miniaturized organoid models of intestinal stem cell differentiation into Paneth cells.

[iScience] The authors found that lysophosphatidic acid induced the differentiation of a previously unsuspected keratinocyte subpopulation expressing the extracellular matrix protein, thrombospondin-1 (THBS1).

[Stem Cell Reviews and Reports] The authors describe the effects of various immune cells on intestinal stem cells (ISCs), as well as the mechanisms underlying these effects and also highlight the remarkable role of ISCs in intestinal pathogenesis.

[Communications Biology] Researchers explored the metabolic reprogramming relevant to lung adenocarcinoma metastasis and decoded the underlying intercellular alterations.