western blot analysis

[Experimental Cell Research] Researchers examined the involvement of advanced glycation end (AGE)-related receptors on AGE-induced angiogenesis in endothelial cells.

[Cell Cycle] Researchers conducted quantitative reverse transcription PCR to find that circ_0004018 was down-regulated in hepatocellular carcinoma cells. Western blot analysis was performed to detect the protein levels of phosphatase and tensin homologue and related factors of PI3K/AKT signaling pathway.

[Acta Biochimica et Biophysica Sinica] Scientists used Caco-2 cell monolayers as an in vitro model of human intestinal epithelial barrier (IEB) to evaluate the toxicity of chlorothalonil exposure on the structure and function of IEB.

[Aging] Scientists screened rutin (RT) for its activity against osteoarthritis with in vivo and in vitro models of osteoarthritis. Pretreatment of RT decreased the ECM degradation, inhibited expression of TRAF-6 and BCL-2 the NF-κB/MAPK pathway proteins.

[Cell Death Discovery] Investigators defined the underlying role of histone deacetylase 4 (HDAC4) and miR-206 in the pathological process of myocardial ischemia-reperfusion injury (MIRI). Up-regulation of HDAC4 and down-regulation of miR-206 occurred in rat myocardial tissues and cardiomyocytes in MIRI.

[Laboratory Investigation] Researchers investigated the possible effect of bioactive glass (BG) on pyroptosis in wound healing both in vivo and in vitro and showed that BG could accelerate wound closure, granulation formation, collagen deposition, and angiogenesis.